MABT65

Anti-CEACAM1 Antibody, clone 4D1/C2

clone 4D1/C2, from mouse

• Sinónimos: Carcinoembryonic antigen-related cell adhesion molecule 1, Biliary glycoprotein 1, BGP-1, CD66a
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• biological source: mouse
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 4D1/C2, monoclonal
• species reactivity: human
• technique(s): ELISA: suitable; flow cytometry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_001020083
• UniProt accession no.: P13688
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... CEACAM1(634)

Descripción

General description

CEACAM1 (carcinoembryonic antigen-related cell adhesion molecule 1) is thought to be a member of the immunoglobulin superfamily and is known as an epithelial tumor suppressor and an angiogenic growth factor. It has also been linked to the actin-based cytoskeleton. CEACAM1 is also known as a cellular receptor for a number of human mucosa pathogenic bacteria. The loss of activity of CEACAM1 has been related to the development of colorectal cancer.

Immunogen

Epitope: Unknown

Recombinant protein corresponding to human CEACAM1.

Application

Detect CEACAM1 using this Anti-CEACAM1 Antibody, clone 4D1/C2 validated for use in WB, IH.

Immunohistochemistry Analysis: A representative lot was used by an independent laboratory to detect CEACAM1 in non-small-cell lung cancer (Dango, S., et al. (2008). Lung Cancer. 60:426-433).

Western Blot Analysis: A representative lot was used by an independent laboratory to detect CEACAM1 in human granulocyte lysate (Bogoevska, V. et al., (2006). Glycobiology. 16(3): 197–209).

Flow Cytometry Analysis: A representative lot was used by an independent laboratory to detect CEACAM1 in human granulocytes by FACS analysis (Bogoevska, V. et al., (2006). Glycobiology. 16(3): 197–209).

Research Category
Cell Structure

Research Sub Category
Adhesion (CAMs)

Quality

Evaluated by Western Blot in HepG2 cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected CEACAM1 in 10 µg of HepG2 cell lysate.

Target description

~150 kDa observed.
Bands are usually observed at ~120-160 kDa, resulting from different glycosylations (Lauke, H., et al. (2004). Mol. Hum. Reprod. 10(4):247-252).

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HepG2 cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable