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Key Documents

MABF291

Sigma-Aldrich

Anti-S100A8/S100A9 Antibody, clone 5.5

clone 5.5, from mouse

Sinónimos:

Protein S100-A9, Calgranulin-B, Calprotectin L1H subunit, Leukocyte L1 complex heavy chain, Migration inhibitory factor-related protein 14, S100 calcium-binding protein A9, AMRP-14, p14, Protein S100-A8, Calgranulin-A, Calprotectin L1L subunit, Cystic fi

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

5.5, monoclonal

species reactivity

human

technique(s)

ELISA: suitable
flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

General description

S100A8, also known as Protein S100-A8, Calgranulin-A, Calprotectin L1L subunit, Cystic fibrosis antigen (CFAG), Leukocyte L1 complex light chain, Migration inhibitory factor related protein 8 (MRP-8), p8, S100 calcium binding protein A8, or Urinary stone protein band A, and encoded by the gene S100A8/CAGA/CFAG/MRP8, is an important protein that binds both zinc and calcium and plays an important role in the regulation of inflammatory processes and immune response. Found in complex with its partner S100A9, S100A8 facilitates chemotaxis, fatty acid trafficking, cytoskeleton reorganization, and NAPDPH oxidase activation and other intracellular activities, as well as a host of extracellular induced activities such as extracellular proinflammatory, antimicrobial, oxidant scavenging, and apoptotic activities . Additionally S100A8 can act as a potent autoimmunity amplifier and appears to augment various cancers and their spread when over expressed. S100A8 is widely expressed and used as biomarker in patients with inflammatory diseases and as a cancer marker in multiple forms of cancer.

Specificity

This antibody detects both S100A8 and S100A9.

Immunogen

Whole cells expressing Human S100A8 & S100A9.

Application

Research Category
Inflammation & Immunology
Research Sub Category
Immunological Signaling
This Anti-S100A8/S100A9 Antibody, clone 5.5 is validated for use in Flow Cytometry and Immunoprecipitation and Immunohistochemistry and ELISA and Western Blotting for the detection of S100A8/S100A9.
Western Blotting Analysis: A representative lot of this antibody was used to detect S100A8/S100A9 in Human monocyte and neutrophil extracts (Edgeworth, J., et al., (1991) JBC. 266(12):7706-7713).
Western Blotting Analysis: A representative lot of this antibody was used to detect S100A8/S100A9 in neutrophil extracts (Hogg et al., 1989).
Immunoprecipitation Analysis: A representative lot of this antibody was used to detect S100A8/S100A9 in Human monocyte and neutrophil lysate (Edgeworth, J., et al., (1991) JBC. 266(12):7706-7713).
Immunoprecipitation Analysis: A representative lot of this antibody was used to detect S100A8/S100A9 in MRP-8 and TL-14 mutant lysate (Hessian P.A., et al., (2001) Eur. J. Biochem. 268:353-363).
Immunohistochemistry Analysis: A representative lot of this antibody was used to detect S100A8/S100A9 in Human Bronchus tissue (Hogg, N., et al., (1989) Eur. J. Immunol. 19:1053-1061).
Immunohistochemistry Analysis: A representative lot of this antibody was used to detect S100A8/S100A9 in spleen and thymus tissue (Hogg, N., et al., (1989) Eur. J. Immunol. 19:1053-1061).
ELISA: A representative lot of this antibody was used to detect S100A8/S100A9 in ELISA (Ryckman, C., et al., (2003) Arthritis & Rheumatism. 48(8):2310-2320).

Quality

Evaluated by Flow Cytometry on Human PBMCs.

Flow Cytometry Analysis: A 1:80 dilution (0.25 µg) of this antibody detected S100A8 and/or S100A9 in 1x10E6 PBMCs.

Target description

~8 kda(11kDa) and 14 kDa and as a heterodimer it would be ~24 kDa (under native conditions)

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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