MABF2156

Anti-TLR9 Antibody, clone NaR9

• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• antibody form: purified antibody
• clone: NaR9; monoclonal
• purified by: using protein G
• species reactivity: mouse, mouse, mouse
• concentration: (Please refer to lot specific datasheet.)
• technique(s): flow cytometry: suitable; immunocytochemistry: suitable
• isotype: IgG2aκ
• UniProt accession no.: Q9EQU3
• mode of action: | inhibits

Descripción

General description

Ba/F3 cells expressing full-length mouse TLR9 protein. Immunization was carried out on Tlr9 -/- mice.

Specificity

Clone NaR9 is a mouse monoclonal antibody that specifically detects Toll-like receptor 9 in murine species. It targets an epitope within the N-terminal region.

Application

Immunocytochemistry Analysis: A representative lot detected TLR9 in Immunocytochemistry applications (Murakami, Y., et. al. (2017). Sci Rep. 7:44042).

Flow Cytometry Analysis: A representative lot detected TLR9 in Flow Cytometry applications (Murakami, Y., et. al. (2017). Sci Rep. 7:44042).

Inhibits Activity/Function: A representative lot inhibited TLR9-dependent cytokine production in vitro by bone marrow-derived macrophages and conventional dendritic cells. (Murakami, Y., et. al. (2017). Sci Rep. 7:44042).

Quality

Evaluated by Immunocytochemistry in Raw264.7 cells.

Immunocytochemistry Analysis: A 1:250 dilution of this antibody detected TLR9 in Raw264.7 cells.

Physical properties

116.41 kDa calculated.

Physical form

Purified mouse monoclonal antibody IgG2a in PBS without azide.

Storage and Stability

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.