MABE889

Anti-ADAR2 Antibody, clone 1.3.1

clone 1.3.1, from mouse

• Sinónimos: Double-stranded RNA-specific editase 1, RNA-editing deaminase 1, RNA-editing enzyme 1, dsRNA adenosine deaminase
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: 1.3.1, monoclonal
• Application: WB
• Citations: 1

Propiedades

• biological source: mouse
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 1.3.1, monoclonal
• species reactivity: human
• technique(s): western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_001103
• UniProt accession no.: P78563
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... ADARB1(104)

Descripción

General description

Double-stranded RNA-specific editase 1, or RNA-editing deaminase 1, or RNA-editing enzyme 1, or dsRNA adensine deaminase, and encoded by the human gene ADARB1/ ADAR2, DRADA2, RED1 is an enzyme that catalyzes the deamination of adenosine in dsRNA to inosine in a step called A to I RNA editing. This editing alters gene expression by changing codon usage and splicing sites as well as altering general RNA stability. So far the enzyme has been shown to edit and destabilize cancer associated genes as well as functional neurotransmitter receptors, and channel proteins. Interestingly, its activity appears to inhibit cell proliferation and migration in some cells and promote exocytosis and metabolism in others, likely to do differential affects on particular important mRNAs. Highly expressed in brain, heart with lower expression in lung, kidney and liver tissues. Interestingly RNA editing correlates with the grade of malignancy of the tumors, with the high grade tumors showing lower editing is seen.

~80 kDa observed

Immunogen

Epitope: C-terminus

GST-tagged recombinant protein corresponding to the C-terminus of human ADAR2.

Application

Anti-ADAR2 Antibody, clone 1.3.1 detects level of ADAR2 & has been published & validated for use in ADAR2.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Chromatin Biology

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Evaluated by Western Blotting in HeLa nuclear extract.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected ADAR2 in 10 µg of HeLa nuclear extract.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Clase de almacenamiento: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable