AQ502B

Goat Anti-Mouse IgM+IgG+IgA Antibody, F(ab′)2, biotin conjugate, Species Adsorbed

0.5 mg/mL, Chemicon^®

• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.46

Propiedades

• biological source: goat
• Quality Level: 100
• conjugate: biotin conjugate
• antibody form: purified antibody
• antibody product type: secondary antibodies
• clone: polyclonal
• species reactivity: mouse
• manufacturer/tradename: Chemicon^®
• concentration: 0.5 mg/mL
• technique(s): ELISA: suitable; immunofluorescence: suitable
• shipped in: wet ice
• target post-translational modification: unmodified

Descripción

General description

IgG is the most abundant immunoglobulin and is comprised of two heavy chains and two light chains. Each molecule has two antigen binding sites. IgG has 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%). IgM constitutes about 10% of serum immunoglobulins. IgM (with IgD) is the major immunoglobulin expressed on the surface of B cells. Monomeric IgA constitutes 5-15 % of the serum immunoglobulins whereas dimeric IgA is localized to mucosa surfaces.

Specificity

Reacts with heavy and light chains of mouse IgM, IgG1, IgG2a, IgG2b, IgG3 and IgA. Absorbed for human sera and purified human paraproteins. Minimal cross reactivity with human immunoglobulins.

The antibody reacts with the heavy and light chains of mouse IgM, IgG1, IgG2a, IgG2b, IgG3 and IgA as demonstrated by ELISA and flow cytometry. Minimal cross reactivity with human immunoglobulins.

Immunogen

Epitope: Mouse IgM, IgG, IgA

Prepared from pepsin digest of mouse Ig (H+L).

Application

Goat anti-Mouse IgM+IgG+IgA Antibody, F(ab′)2, biotin conjugate, Species Adsorbed detects level of Mouse IgM+IgG+IgA & has been published & validated for use in ELISA & IF.

Immunofluorescence: ≤ 1 μg/10⁶ cells.
ELISA: 1:5,000-1:20,000.

Optimal working dilutions must be determined by the end user.

Research Category
Secondary & Control Antibodies

Research Sub Category
Fragment Specific Secondary Antibodies

Physical form

Gel filtration chromatography

Purified by gel filtration chromatography of pepsin digested antibody. Liquid in 1.0 mL PBS/NaN₃.

Storage and Stability

Maintain refrigerated at 2°-8°C under sterile conditions for up to twelve months from date of receipt. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable