Centrifuge at 500 x g for 5 min.
69203
Streptavidin Agarose
Column or batch isolation of biotinylated molecules
Sinónimos:
Streptavidin beads
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Acerca de este artículo
NACRES:
NA.56
UNSPSC Code:
41106500
Form:
liquid
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Servicio técnico
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Permítanos ayudarleQuality Segment
form
liquid
manufacturer/tradename
Novagen®
storage condition
do not freeze
shipped in
ambient
storage temp.
2-8°C
General description
| Specifications | |
| Concentration | 50% suspension (5 ml settled volume) |
| Storage buffer | 100 mM sodium phosphate pH 7.5, 0.02% sodium azide |
| Size | 40-165 µm |
| Binding capacity | >85 nmol free biotin/ml |
| Protease | None detected (SDS-PAGE of purified protein) |
| DNase | None detected (blue/white colony assay of ligated plasmid) |
| RNase | None detected (gel assay of purified RNA) |
Column or batch isolation of biotinylated molecules
Streptavidin Agarose consists of cross-linked agarose covalently coupled with pure streptavidin under controlled conditions. The stable linkage to the resin minimizes leaching of the streptavidin while maintaining full binding activity. The matrix is suitable for use in column and batch formats for any application that requires high biotin binding capacity and low non-specific binding. The resin has no detectable protease, DNase, or RNase contamination. Provided as a 50% slurry in storage buffer; 5 ml settled resin in 10 ml total volume.
Legal Information
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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Este artículo | |||
|---|---|---|---|
| description Column or batch isolation of biotinylated molecules | description suitable for affinity chromatography | description streptavidin 1-2 units/mg protein, lyophilized powder | description For purification of biotinylated macromolecules and complexes |
| Quality Level 100 | Quality Level 100 | Quality Level 200 | Quality Level - |
| form liquid | form suspension | form lyophilized powder | form aqueous suspension |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. 2-8°C |
| storage condition do not freeze | storage condition - | storage condition - | storage condition - |
| shipped in ambient | shipped in - | shipped in - | shipped in - |
| manufacturer/tradename Novagen® | manufacturer/tradename - | manufacturer/tradename - | manufacturer/tradename - |
Clase de almacenamiento
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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What is the recommended centrifuge speed for this product regarding biotinylated protein purification (especially washing steps)?
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What is the total mass of the agarose in the 5ML pack size of product 69203-3, Streptavidin Agarose?
1 answer-
This product is provided as a 50% slurry, reflecting volume-to-volume ratio. The amount of streptavidin agarose used is strictly based on volume and not by its weight.
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