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426205

β-Lactamase, Bacillus cereus 569/H9

Name: β-Lactamase, <i>Bacillus cereus</i> 569/H9
Brand: MM

Sinónimos:

β-Lactamase, Bacillus cereus 569/H9, Penicillinase

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Número CAS:

9073-60-3

Número CE:

3.5.2.6 (BRENDA, IUBMB)

UNSPSC Code:

12352202

NACRES:

NA.54

MDL number:

MFCD00131819

Biological source:

Bacillus sp. (Bacillus cereus)

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Propiedades

biological source

Bacillus sp. (Bacillus cereus)

Quality Segment

100

description

Merck USA index - 14, 7093

form

lyophilized

packaging

vial of ≥50 units β-lactamase II, vial of ≥500 units β-lactamase I

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze

solubility

sterile distilled water: soluble

shipped in

ambient

storage temp.

−20°C

Descripción

General description

β-Lactamase produced by bacteria shares sequence homology to penicillin-binding proteins. This enzyme is found in Gram-negative bacteria. β-Lactamases have four molecular classes, named A, B, C, and D. A, C, and D form an acyl-enzyme via active site serine residue. Class B β-lactamases are metalloenzymes, which have a zinc ion at their active site for β-lactam hydrolysis.

Biochem/physiol Actions

β-Lactamase hydrolyzes β-lactum antibiotics and is the chief cause of resistance to β-lactam antibiotics development by bacteria. Mutations in the β-lactamases are associated with extended-spectrum β-lactamases (ESBLs).

Preparation Note

Following reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 1 month at 4°C.

Other Notes

One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol benzyl penicillin and 1.0 µmol of cephalosporin C, respectively, per min at 25°C.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Harmful (C)

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Este artículo	P4524	61305	P0389
Sigma-Aldrich 426205 β-Lactamase, Bacillus cereus 569/H9	Sigma-Aldrich P4524 β-Lactamase from Enterobacter cloacae	Sigma-Aldrich 61305 Penicillinase from Bacillus cereus	Sigma-Aldrich P0389 Penicillinase from Bacillus cereus
description -	description Type IV, lyophilized powder, 0.2-0.6 units/mg protein (using benzylpenicillin)	description lyophilized, powder, white, ~13 U/mg	description lyophilized powder, ≥1500.00 units/mg protein (using benzylpenicillin)
biological source Bacillus sp. (Bacillus cereus)	biological source bacterial (Enterobacter cloacae)	biological source Bacillus sp. (Bacillus cereus)	biological source Bacillus sp. (Bacillus cereus)
form lyophilized	form lyophilized powder	form powder	form lyophilized powder
shipped in ambient	shipped in -	shipped in -	shipped in wet ice
solubility sterile distilled water: soluble	solubility -	solubility -	solubility -
storage temp. −20°C	storage temp. 2-8°C	storage temp. −20°C	storage temp. 2-8°C
storage condition OK to freeze	storage condition -	storage condition -	storage condition -

pictograms

GHS08

signalword

Danger

hcodes

H317,H334

pcodes

P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c