189730
Streptavidin
Sinónimos:
Streptavidin, Avidin, Streptomyces avidinii
Iniciar sesión para ver los precios por organización y contrato.
Seleccione un Tamaño
Cambiar Vistas
| Talla/SKU | Disponibilidad | Precio |
|---|
Acerca de este artículo
Form:
lyophilized solid
Biological source:
(Streptomyces avidinii)
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Servicio técnico
¿Necesita ayuda? Nuestro equipo de científicos experimentados está aquí para ayudarle.
Permítanos ayudarlebiological source
(Streptomyces avidinii)
Quality Segment
form
lyophilized solid
specific activity
≥10 units/mg protein
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, desiccated
shipped in
ambient
storage temp.
−20°C
General description
A high affinity biotin binding protein (Kd approximately 10-15) that is stable over wide pH and temperature ranges. Each molecule of streptavidin binds 4 molecules of biotin. Streptavidin lacks the carbohydrate side chains present on avidin and has an isoelectric point nearer to neutrality (pI of 5.5-6.5 versus 10 for avidin). Consequently, streptavidin frequently exhibits lower levels of non-specific binding than avidin. The extinction coefficient of a 1% solution at 280 nm is 32.
A high-affinity biotin binding protein.
Preparation Note
Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short-term storage. Aqueous stock solutions are stable for 6 months at 4°C and for up to 2 years at -20°C.
Reconstitute in H₂O or 1 mM NaHCO₃, pH 9.0 at twice the final concentration and then dilute with an equal volume of 2X buffer.
Other Notes
Eberle, J. and Seibl, R. 1992. J. Virol. Methods 40, 347.
Gudibande, S.R., et al. 1992. Mol. Cell. Probes. 6, 495.
Nesbitt, S.A. and Horton, M.A. 1992. Anal. Biochem. 206, 267.
Roth, J., et al. 1992. Histochemistry 98, 229.
Sano, T., et al. 1992. Science 258, 120.
Scouten, W.H. and Konecny, P. 1992. Anal. Biochem. 205, 313.
Srivastava, P., et al. 1992. Cytometry 13, 711.
Gudibande, S.R., et al. 1992. Mol. Cell. Probes. 6, 495.
Nesbitt, S.A. and Horton, M.A. 1992. Anal. Biochem. 206, 267.
Roth, J., et al. 1992. Histochemistry 98, 229.
Sano, T., et al. 1992. Science 258, 120.
Scouten, W.H. and Konecny, P. 1992. Anal. Biochem. 205, 313.
Srivastava, P., et al. 1992. Cytometry 13, 711.
One unit is defined as the amount of streptavidin required to bind 1.0 µg of D-Biotin at pH 7.0.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
1 of 1
Este artículo | |||
|---|---|---|---|
| description - | description recombinant, expressed in E. coli, lyophilized powder | description - | description For purification of biotinylated macromolecules and complexes |
| biological source (Streptomyces avidinii) | biological source - | biological source chicken | biological source - |
| form lyophilized solid | form lyophilized powder | form lyophilized solid | form aqueous suspension |
| specific activity ≥10 units/mg protein | specific activity - | specific activity - | specific activity - |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C |
| shipped in ambient | shipped in - | shipped in ambient | shipped in - |
| storage condition desiccated, OK to freeze | storage condition - | storage condition OK to freeze | storage condition - |
Still not finding the right product?
Explore all of our products under Streptavidin
Clase de almacenamiento
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Elija entre una de las versiones más recientes:
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.