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F2178

Sigma-Aldrich

Anti-FKHRL1 (FOXO3a) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-AF6q21, Anti-FKHRL1, Anti-FKHRL1P2, Anti-FOXO2, Anti-FOXO3A

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

mouse, human

packaging

antibody small pack of 25 μL

technique(s)

indirect immunofluorescence: 1:200 using paraformaldyde-fixed FKHRL1 transfected 293T cells
microarray: suitable
western blot: 1:2,500 using human FKHRL1 in transfected 293T cell extracts

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... FOXO3(2309)
mouse ... Foxo3(56484)

General description

Forkhead family transcription factor (FKHRL1), also known as FOX3a, is a member of the forkhead transcription factor superfamily. In FKHRL1 (FOX3a), there are three PKB phosphorylation consensus sites, Thr32, Ser253, and Ser315. Thr32 and Ser253 are phosphorylated by PKB after induction by survival factors such as IGF-1.

Immunogen

thetic peptide corresponding to the N-terminus of human FKHRL1 (amino acids 653-668 with N-terminally added cystein) conjugated to maleimide-activated KLH. This sequence is conserved in human and mouse FKHRL1.

Application

Anti-FKHRL1 (FOXO3a) antibody produced in rabbit has been used in:
  • immunohistochemistry
  • western blotting
  • immunofluorescence staining

Biochem/physiol Actions

Forkhead transcription factor superfamily proteins act as the targets of the phosphoinositide-3-kinase–protein kinase B/Akt (PI3K-PKB) pathway. FKHRL1/FOXO3a modulates the expression of several genes that regulate DNA repair in response to stress at the G2-M checkpoint and aging. Other genes that are regulated by FKHRL1 include mitotic genes such as cyclin B and polo-like kinase (plk). Antibodies reacting specifically with FKHRL1 (FOXO3) may be useful in studying the expression and function of the protein, as well as for correlating their expression pattern with physiological functions or pathological conditions.
Forkhead1 (FOXO-3A or FKHRL-1) functions to protect cells from oxidative stress . Dephosphorylation of FOXO3a stimulates the Fas ligand gene that activates apoptosis in cells . Anti-FKHRL1 is specific for human and mouse FKHRL1. FKHRL1 immunizing peptide specifically inhibits the staining of FKHRL1.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin, and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Protein Kinase SGK Mediates Survival Signals by Phosphorylating the Forkhead Transcription Factor FKHRL1 (FOXO3a)
Brunet A, et al.
Molecular and Cellular Biology, 21(3), 952-965 (2001)
The Role of Foxo3 in Leydig Cells
Choi YS, et al.
Yonsei Medical Journal, 56(6), 1590-1590 (2015)
Forkhead transcription factors contribute to execution of the mitotic programme in mammals
Alvarez, B, et al.
Nature, 413(6857), 744-747 (2001)
Novel Hsp90 inhibitor FW-04-806 displays potent antitumor effects in HER2-positive breast cancer cells as a single agent or in combination with lapatinib
Huang W, et al.
Cancer Letters, 356(2), 862-871 (2015)
FOXO3 Knockdown Accelerates Development of Bovine Primordial Follicles
BAO RM, et al.
Journal of Reproduction and Development, 57(4), 475-480 (2011)

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