HPLC Analysis of B-Vitamins in Vitamin Water using Ascentis® Express RP-Amide and UV Detection
Materiali
Colonna analitica
N° Catalogo
Descrizione
Determinazione del prezzo
Colonna per HPLC Ascentis® Express RP-Amide, 2,7 μm
2.7 μm particle size, L × I.D. 10 cm × 3 mmCONDITIONS
column
Ascentis Express RP-Amide, 10 cm x 3.0 mm I.D., 2.7 μm particles (53918-U)
mobile phase
[A] 20 mM potassium phosphate, pH 3; [B] methanol
gradient
held at 0.5% B for 1.5 minutes, to 25% B in 1.0 minutes, held at 25% B for 1.7 minute, held at 0.5% B for 1.2 minutes
flow rate
0.75 mL/min
pressure
5584 psi (385 bar)
column temp.
30 °C
detector
UV, 210 nm
injection
0.5 μL
sample
vitamin water was filtered through 0.2um nylon syringe filter
Descrizione
Risultati analitici
The vitamin water drink was filtered using 0.2 um Nylon filter and injected into UHPLC for B-vitamin analysis
Note legali
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany