Passa al contenuto
Merck

HPLC Analysis of Plasmids on TSKgel® DNA-NPR

HPLC Analysis of Plasmids on TSKgel® DNA-NPR application for HPLC

Materiali

Colonna analitica

N° Catalogo
Descrizione
Determinazione del prezzo

TSKgel® DNA-NPR HPLC Column

phase DEAE (diethylaminoethyl), L × I.D. 7.5 cm × 4.6 mm, 2.5 μm particle size

Componente della fase mobile

N° Catalogo
Descrizione
Determinazione del prezzo

Sodio cloruro

for molecular biology, DNase, RNase, and protease, none detected, ≥99% (titration)

Trizma® cloridrato

>99% (titration), pH 7.0-9.0, suitable for FISH

Precolonna

N° Catalogo
Descrizione
Determinazione del prezzo

TSKgel® DEAE-NPR Guard Column

phase DEAE (diethylaminoethyl), L × I.D. 0.5 cm × 4.6 mm, 2.5 μm particle size

CONDITIONS

column

TSKgel DNA-NPR, 7.5 cm x 4.6 mm, 2.5 μm particles (818249)

mobile phase

[A] 0.02 M Tris, pH 9.0; [B] 0.02 M Tris, 1.0 M NaCl, pH 9.0

gradient

50 to 60% B in 10 column volumes

flow rate

1 mL/min

detector

UV, 260 nm

sample

PUC19 plasmid

Descrizione

Altre note

Data provided by Tosoh Bioscience LLC.

Note legali

TSKgel is a registered trademark of Tosoh Corporation