HPLC Analysis of Peptides on Discovery^® BIO Wide Pore C18, Preparative Scale

column

Discovery BIO Wide Pore C18, 15 cm × 10 mm I.D., 10 μm particles (567208-U)

mobile phase

[A] (80:20), 0.1% TFA in water:0.1% TFA in acetonitrile; [B] (66:34), 0.1% TFA in water:0.1% TFA in acetonitrile

flow rate

4.7 mL/min

column temp.

30 °C

detector

UV, 215 nm

injection

24.5 μL

sample

Cat. No. P2693