HPLC Analysis of HSA Fusion Protein on Zenix®-C SEC-300 vs. Zenix® SEC-300
CONDITIONS
column
Zenix SEC-300, 30 cm x 7.8 mm I.D., 3 μm particles (Z777033)
column
Zenix-C SEC-300, 30 cm x 7.8 mm I.D., 3 μm particles (Z777092)
mobile phase
150 mM sodium phosphate buffer, pH 7.0
flow rate
1 mL/min
column temp.
25 °C
detector
UV, 280 nm
injection
10 μL
sample
HSA fusion peptide 5 mg/mL (MW 75 kD, pl 5.0, HSA conjugated peptide in diabetes treatment)
Descrizione
Note legali
Zenix is a registered trademark of Sepax Technologies