HPLC Analysis of FMOC-Isoleucine Enantiomers on Astec^® CHIROBIOTIC^® T

column

CHIROBIOTIC T, 25 cm x 4.6 mm I.D., 5 μm particles (12024AST)

mobile phase

[A] 20 mM ammonium acetate, pH 4.1; [B] methanol; (60:40, A:B)

flow rate

1 mL/min

column temp.

25 °C

detector

UV, 260 nm

injection

3 μL

sample

1 mg/mL in methanol