HPLC Analysis of Coumachlor Enantiomers on Astec^® CYCLOBOND I 2000 DM

column

CYCLOBOND I 2000 DM, 25 cm x 4.6 mm I.D., 5 μm particles (20924AST)

mobile phase

[A] acetonitrile; [B] 20 mM ammonium acetate, pH 2.9; (20:80, A:B)

flow rate

0.8 mL/min

column temp.

25 °C

detector

UV, 230 nm

injection

3 μL

sample

coumachlor, 1 mg/mL in acetonitrile:water (50:50)