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HPLC Analysis of B-Trichothecenes on Ascentis® Express C18

HPLC Analysis of B-Trichothecenes on Ascentis® Express C18 application for HPLC

Materiali

Colonna analitica

N° Catalogo
Descrizione
Determinazione del prezzo

53814-U

Colonna per HPLC Ascentis® Express C18, 2,7 μm

2.7 μm particle size, L × I.D. 10 cm × 3 mm

Standard

N° Catalogo
Descrizione
Determinazione del prezzo

34134

B-Trichothecene mix, (DON, NIV, 3-AcDON, 15-AcDON)

~100 μg/mL in acetonitrile (each of DON, NIV, 3-AcDON and 15-AcDON), analytical standard

CONDITIONS

column

Ascentis Express C18, 10 cm x 3 mm I.D., 2.7 μm particles (53814-U)

mobile phase

[A] water:acetonitrile:methanol (92:4:4); [B] acetonitrile

gradient

0% B held for 5 minutes, to 8% B in 3 minutes, 8% B held for 2 minutes

flow rate

0.8 mL/min

pressure

4061 psi (280 bar)

column temp.

35 °C

detector

UV, 220 nm

injection

40 μL

sample

2 ppm each in water:methanol:acetonitrile (92:4:4)

Descrizione

Risultati analitici

Mycotoxins are toxic secondary metabolites produced by fungi. Those chemicals exist in food as a result of fungal infection of crops. Mycotoxins have strong resistance to decomposition and digestion, so they remain in the food chain in meat and dairy products. They also resist to temperature, such as cooking and freezing. Their effects on human and animal health include death, cancer, weakened immune systems and as allergens or irritants.

Note legali

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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