HPLC Analysis of Alloisoleucine Enantiomers on Astec® CHIROBIOTIC® R
CONDITIONS
column
CHIROBIOTIC R, 25 cm x 4.6 mm I.D., 5 μm particles (13024AST)
mobile phase
[A] water; [B] methanol; (40:60, A:B)
flow rate
0.6 mL/min
column temp.
25 °C
detector
UV, 210 nm
injection
10 μL
sample
1 mg/mL in methanol
Descrizione
Note legali
Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC