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565348-U

Supelco

Ascentis® RP-Amide (5 µm) HPLC Columns

L × I.D. 25 cm × 21.2 mm, HPLC Column

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About This Item

Codice UNSPSC:
41115700
eCl@ss:
32110501
NACRES:
SB.52

product name

Ascentis® RP-Amide HPLC Column, 5 μm particle size, L × I.D. 25 cm × 21.2 mm

Materiali

stainless steel column

agenzia

suitable for USP L60

Nome Commerciale

Ascentis®

Caratteristiche

endcapped

Produttore/marchio commerciale

Ascentis®

Confezionamento

1 ea of

Grado di funzionalizzazione

19.5% Carbon loading

Parametri

≤70 °C temp. range
400 bar pressure (5801 psi)

tecniche

HPLC: suitable

Lungh. × D.I.

25 cm × 21.2 mm

Area superficiale

450 m2/g

Copertura della superficie

2.7 μmol/m2

Impurezze

<5 ppm metals

Matrice

fully porous particle
silica gel high purity, spherical

Gruppo funzionale matrice

amide, alkyl phase

Dimensione particelle

5 μm

Dimensione pori

100 Å

operating pH range

2-8

applicazioni

food and beverages

Tecnica di separazione

reversed phase

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Descrizione generale

The Ascentis family of columns is the fourth generation of HPLC column technology from Supelco scientists. Ascentis columns are bonded on high purity, 100 Angstrom silica including 3, 5, and 10 micron particle size. Columns are designed for small molecule applications and are scalable from micro columns (1.0 mm I.D.) to preparative dimensions (50 mm I.D.). The family includes C18, C8, Phenyl, Si and embedded polar group phase, RP-Amide.

Ascentis RP-Amide is a new generation ultra low bleed, embedded polar group (EPG) phase that provides orthogonal selectivity and increased resolution for HPLC and LC-MS analysis of polar compounds. The Ascentis RP-Amide is the first choice in embedded polar group HPLC phases.

Caratteristiche e vantaggi

  • Excellent retention and peak shape for polar compounds
  • 100% aqueous compatibility
  • Ultra low bleed, LC-MS compatible
  • Unique selectivity

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Note legali

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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Certificati d'analisi (COA)

Lot/Batch Number
253940-01
252446-01
177169-01
176982-01
176776-01

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Jakub Fibigr et al.
Journal of pharmaceutical and biomedical analysis, 151, 291-300 (2018-02-08)
The presented work describes the development and validation of a rapid UHPLC-UV method using a fused core particle column with an RP-Amide stationary phase for the separation and quantitative analysis of caffeoylquinic and di-caffeoylquinic acids in green coffee extracts. Three
Rihana Parveen Shaik et al.
Journal of pharmaceutical analysis, 3(6), 489-499 (2013-12-01)
Liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was used for simultaneous quantification of tolterodine and its metabolite 5-hydroxy methyl tolterodine in rat plasma. Tolterodine-d6 and 5-hydroxy methyl tolterodine-d14 were used as internal standards (IS). Chromatographic separation was performed on Ascentis Express

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