Documenti fondamentali

Visualizza tutta la documentazione

Vai a

T7902

Thermolysin from Geobacillus stearothermophilus

Name: Thermolysin from <I>Geobacillus stearothermophilus</I>
Brand: SIGMA
Price: 66.7 CHF

powder, BioReagent, 30-350 units/mg protein (E1%/280), suitable for cell culture

Sinonimo/i:

Protease from Geobacillus stearothermophilus, Thermophilic-bacterial protease

Autenticati per visualizzare i prezzi organizzativi e contrattuali.

Scegli un formato

Cambia visualizzazione

Voi/SKU	Disponibilità	Prezzo
25 mg	Per conoscere la disponibilità, visualizza il carrello	CHF 66.70
100 mg	Per conoscere la disponibilità, visualizza il carrello	CHF 209.00
250 mg	Per conoscere la disponibilità, visualizza il carrello	CHF 2150.00

Informazioni su questo articolo

Numero CAS:

9073-78-3

UNSPSC Code:

12352204

NACRES:

NA.75

EC Number:

232-973-4

MDL number:

MFCD00165549

Numero CE:

3.4.24.27 (BRENDA, IUBMB)

eCl@ss:

32160410

Specific activity:

30-350 units/mg protein (E1%/280)

Biological source:

Geobacillus stearothermophilus

CHF 66.70

Per conoscere la disponibilità, visualizza il carrello

Richiedi un ordine personalizzato

Servizio Tecnico

Hai bisogno di aiuto? Il nostro team di scienziati qualificati è a tua disposizione.

Permettici di aiutarti

Proprietà

biological source

Geobacillus stearothermophilus

Quality Segment

200

product line

BioReagent

form

powder

specific activity

30-350 units/mg protein (E1%/280)

mol wt

34.6 kDa

purified by

crystallization

technique(s)

cell culture | mammalian: suitable, single cell analysis: suitable

storage temp.

−20°C

Descrizione

Application

A thermostable (thermophilic) extracellular metalloendopeptidase containing four calcium ions. Cofactors are zinc and calcium. Hydrolyzes protein bonds on the N-terminal side of hydrophobic amino acid residues. The pH optimum is 8.0 and the optimal temperature for activity is 70 °C. Considerably stable from pH 5 to 9.5. Thermolysin has a low cleavage specificity, therefore, it produces a number of short fragments that are suitable for sequencing. Preferential cleavage: X-cleavage-Y-Z where X=any amino acid; Y=Leu, Phe, Ile, Val, Met, Ala and Z is any amino acid other than Pro. Cleavage N-terminal to Leu is preferred over cleavage of N-terminal to Phe which is preferred over the others. Often used to do limited proteolysis for peptide mapping and studies of protein structure and conformational changes.

Physical form

lyophilized powder containing calcium and sodium acetate buffer salts

Other Notes

One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).

Confronta articoli simili

Visualizza il confronto per intero

Mostra le differenze

1 of 1

Questo articolo	P1512	C9722	T9935
Sigma-Aldrich T7902 Thermolysin from Geobacillus stearothermophilus	Sigma-Aldrich P1512 Thermolysin from Geobacillus stearothermophilus	Sigma-Aldrich C9722 Collagenasi	Sigma-Aldrich T9935 Tripsina
description powder, BioReagent, 30-350 units/mg protein (E1%/280), suitable for cell culture	description Type X, lyophilized powder, 30-350 units/mg protein (E1%/280)	description lyophilized powder (from 0.2μm filtered solution), 0.5-5.0 FALGPA units/mg solid, suitable for cell culture	description essentially salt-free, lyophilized powder, ≥9,000 BAEE units/mg protein, BioReagent, suitable for cell culture
specific activity 30-350 units/mg protein (E1%/280)	specific activity 30-350 units/mg protein (E1%/280)	specific activity 0.5-5.0 FALGPA units/mg solid	specific activity ≥9,000 BAEE units/mg protein
biological source Geobacillus stearothermophilus	biological source Geobacillus stearothermophilus	biological source Clostridium histolyticum	biological source -
technique(s) cell culture \| mammalian: suitable, single cell analysis: suitable	technique(s) -	technique(s) cell culture \| mammalian: suitable, single cell analysis: suitable	technique(s) cell culture \| mammalian: suitable
form powder	form lyophilized powder	form lyophilized powder (from 0.2μm filtered solution)	form essentially salt-free, lyophilized powder
mol wt 34.6 kDa	mol wt 34.6 kDa by amino acid sequence	mol wt -	mol wt 23.8 kDa
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C

Still not finding the right product?

Explore all of our products under Thermolysin from Geobacillus stearothermophilus

Parola di avvertenza

Danger

Classe di stoccaggio

11 - Combustible Solids

Punto di infiammabilità (°F)

Not applicable

Punto di infiammabilità (°C)

Not applicable

DPI (dispositivi di protezione individuale)

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Organi bersaglio

Respiratory system

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Che succede?

WGK 1

Scegli una delle versioni più recenti:

Certificati d'analisi (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documenti section.

Se ti serve aiuto, non esitare a contattarci Servizio Clienti

Possiedi già questo prodotto?

I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.