SRP3300

HGF mouse

recombinant, expressed in Hi-5 Insect cells, ≥95% (SDS-PAGE), ≥95% (HPLC)

• Sinonimo/i: Hepatopoietin (HPTA), Scatter Factor (SF)
• Codice UNSPSC: 12352202
• NACRES: NA.32

Proprietà

• Origine biologica: mouse
• Ricombinante: expressed in Hi-5 Insect cells
• Saggio: ≥95% (HPLC); ≥95% (SDS-PAGE)
• Stato: lyophilized
• Potenza: 10-20 ng/mL
• PM: 80.0 kDa
• Confezionamento: pkg of 20 μg
• Impurezze: <0.1 EU/μg endotoxin, tested
• Colore: white to off-white
• N° accesso UniProt: Q08048
• Condizioni di spedizione: wet ice
• Temperatura di conservazione: −20°C
• Informazioni sul gene: mouse ... HGF(15234)

Descrizione

Descrizione generale

Hepatocyte growth factor (HGF) is a plasmin related disulfide-linked, hetereodimeric protein containing an α and a β chain. It is synthesized as inactive, single-chain precursor which shares similarity with plasminogen. Its α chain contains an N-terminal hairpin and four kringle domains (NK4).[1]

Azioni biochim/fisiol

Hepatocyte growth factor (HGF) functions via its receptor c-Met, which gets activated upon tyrosine phosphorylation, and subsequently promotes epithelial cell proliferation, morphogenesis, angiogenesis and motility. It plays a crucial role in organ development, as inactivation of either this gene or its receptor in fetal stages results in organ hypoplasia. Endogenously this protein is involved in the self-repair of various ograns including injured livers, kidneys, lungs etc.[2] HGF is a predominant potent factor involved in lung growth and repair. It functions as a motogen, tubulogen and anti-apoptotic agent on airway epithelial and other cells expressing HGF receptor c-Met.[1] Studies in mice show that HGF determines the severity of Pseudomonas aeruginosa keratitis by enhanced HGF signaling through c-met resulting in elevated pro-inflammatory cytokines and reduced anti-inflammatory cytokines.[3]

Stato fisico

Lyophilized from 10 mM Tris, pH 7.2 + 100mM L-Arginine + 200 mM NaCl.

Ricostituzione

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Note: Slow to dissolve. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable