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Key Documents

SRP2104

Sigma-Aldrich

Arginine Splicing Factor, His tagged human

recombinant, expressed in insect cells, ≥85% (SDS-PAGE)

Sinonimo/i:

ASF, FLJ53078, MGC5228, SF2, SF2p33, SFRS1, SRp30a

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About This Item

Codice UNSPSC:
12352202
NACRES:
NA.26

Origine biologica

human

Ricombinante

expressed in insect cells

Saggio

≥85% (SDS-PAGE)

Forma fisica

frozen liquid

PM

~29.3 kDa

Confezionamento

pkg of 5 μg

Condizioni di stoccaggio

avoid repeated freeze/thaw cycles

Concentrazione

350 μg/mL

Colore

clear
 colorless

N° accesso NCBI

NM_006924

N° accesso UniProt

Q07955

Condizioni di spedizione

dry ice

Temperatura di conservazione

−70°C

Informazioni sul gene

human ... SRSF1(6426)

Azioni biochim/fisiol

ASF also known as SF2 is a member of the SR protein family. It is an essential pre-mRNA splicing factor required for both single and alternative splicing. Phosphorylation on serine residues located within the SR domain directly regulates ASF/SF2 activity and compartmentalization of other SR splicing factors. In addition to interacting with RNA and other splicing factors, such as U1-70K, U2AF and other SR proteins, ASF/SF2 also directly or indirectly interacts with HIV regulatory protein Rev, the C-terminal domain (CTD) of the largest subunit of RNA polymerase II, and numerous transcription factors, thereby suggesting a potential role of ASF/SF2 in coordinating transcription and pre-mRNA splicing.

Stato fisico

Clear and colorless frozen liquid solution

Nota sulla preparazione

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

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A R Krainer et al.
Cell, 66(2), 383-394 (1991-07-26)
SF2 is a protein factor essential for constitutive pre-mRNA splicing in HeLa cell extracts and also activates proximal alternative 5' splice sites in a concentration-dependent manner. This latter property suggests a role for SF2 in preventing exon skipping, ensuring the
H Ge et al.
Cell, 62(1), 25-34 (1990-07-13)
SV40 early pre-mRNA is alternatively spliced by utilization of two different 5' splice sites and a shared 3' splice site to produce large T and small t mRNAs. The ratio of small t to large T mRNAs produced in human
A M Zahler et al.
Genes & development, 6(5), 837-847 (1992-05-01)
We demonstrate that four different proteins from calf thymus are able to restore splicing in the same splicing-deficient extract using several different pre-mRNA substrates. These proteins are members of a conserved family of proteins recognized by a monoclonal antibody that

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