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Documenti fondamentali

SRP2096

Sigma-Aldrich

p300-Ch1 (302-531) human

recombinant, expressed in E. coli, ≥80% (SDS-PAGE)

Sinonimo/i:

KAT3B, RP1-85F18.1, RSTS2, p300

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About This Item

Codice UNSPSC:
12352200
NACRES:
NA.26

Origine biologica

human

Ricombinante

expressed in E. coli

Saggio

≥80% (SDS-PAGE)

Stato

frozen liquid

PM

~26 kDa

Confezionamento

pkg of 10 μg

Concentrazione

1000 μg/mL

Colore

clear colorless

N° accesso NCBI

N° accesso UniProt

Condizioni di spedizione

dry ice

Temperatura di conservazione

−70°C

Informazioni sul gene

human ... EP300(2033)

Descrizione generale

The EP300 (E1A binding protein p300) gene is mapped to human chromosome 22q13.2. It codes for a histone acetyltransferase which is ubiquitously expressed.

Azioni biochim/fisiol

Human p300 and CBP (CREB binding protein) are highly related transcriptional coactivators. Both proteins have been identified through protein interaction assays. In addition to interacting with variety of cellular factors and onco-proteins, loss of the wild type CBP alleles in isolated tumors suggests that CBP/p300 might serve as tumor suppressors. The ability of p300 to acetylate many transcription factors, including p53, E2F, TFIIE, and TFIIF etc. demonstrated a novel mechanism of targeted p300 regulation of gene expression.
Loss of EP300 (E1A binding protein p300) gene function causes Rubinstein-Taybi syndrome. p300 is essential for cell differentiation, growth and survival. p300 promotes liver stiffness, which is known to induce hepatic stellate cell differentiation to tumor-promoting myofibroblasts.

Stato fisico

Clear and colorless frozen liquid solution

Nota sulla preparazione

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


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Genome-wide sequencing expands the phenotypic spectrum of EP300 variants
Costain G, et al.
European Journal of Medical Genetics, 61(3), 125-129 (2018)
P300 acetyltransferase mediates stiffness-induced activation of hepatic stellate cells into tumor-promoting myofibroblasts
Dou C, et al.
Gastroenterology, 154(8), 2209-2221 (2018)
Dysregulation of Sirtuin 2 (SIRT2) and histone H3K18 acetylation pathways associates with adverse prostate cancer outcomes
Damodaran S, et al.
BMC Cancer, 17(1), 874-874 (2017)
Jeroen H Roelfsema et al.
American journal of human genetics, 76(4), 572-580 (2005-02-12)
CREB-binding protein and p300 function as transcriptional coactivators in the regulation of gene expression through various signal-transduction pathways. Both are potent histone acetyl transferases. A certain level of CREB-binding protein is essential for normal development, since inactivation of one allele
R Eckner et al.
Genes & development, 8(8), 869-884 (1994-04-15)
The growth-controlling functions of the adenovirus E1A oncoprotein depend on its ability ot interact with a set of cellular proteins. Among these are the retinoblastoma protein, p107, p130, and p300. We have isolated a cDNA encoding full-length human p300 and

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