SRP0243

aRAF Active human

recombinant, expressed in baculovirus infected insect cells, ≥80% (SDS-PAGE)

• Sinonimo/i: A-Raf proto-oncogene, PKS2, v-Raf murine sarcoma 3611 viral oncogene homolog
• Codice UNSPSC: 12352202
• NACRES: NA.32

Proprietà

• Origine biologica: human
• Ricombinante: expressed in baculovirus infected insect cells
• Saggio: ≥80% (SDS-PAGE)
• Forma fisica: aqueous solution
• PM: 63.6 kDa
• Confezionamento: pkg of 10 μg
• Condizioni di stoccaggio: avoid repeated freeze/thaw cycles
• Concentrazione: >0.02 mg/mL
• N° accesso NCBI: NM_001654
• N° accesso UniProt: P10398
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −70°C
• Informazioni sul gene: human ... ARAF(369)

Descrizione

Descrizione generale

Human A-RAF (GenBank Accession No. NM_001654), amino acids 282-end, containing the mutations YY301-302DD, MW = 63.6 kDa, with N-terminal GST tag, expressed in an Baculovirus infected Sf9 cell expression system.

Applicazioni

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Stato fisico

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, and 3 mM DTT.

Nota sulla preparazione

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.