Too much residual medium or cell mass may lead to recovery of some large RNAs. It is critical to remove as much residual medium as possible by re-centrifuging the pellet. One can also test different ratios of the lysis mix (Small RNA Lysis Buffer and Binding Solution).
SNC10
Mission® Small RNA Purification Kit
1 sufficient for 10 preparations
Sinonimo/i:
microRNA Isolation Kit
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1 KIT
CHF 102.00
CHF 102.00
Spedizione prevista il01 giugno 2025
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1 KIT
CHF 102.00
About This Item
Codice UNSPSC:
41105501
NACRES:
NA.52
CHF 102.00
Spedizione prevista il01 giugno 2025
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impiego
1 sufficient for 10 preparations
tecniche
DNA extraction: suitable
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Descrizione generale
Sigma′s mirPremier microRNA Isolation Kit provides a rapid and efficient method for purifying and enriching miRNAs and other small RNAs from diverse biological sources, including mammalian cell cultures, animal tissues, plant tissues, and microbial cultures, without using hazardous organic extractions. microRNAs (miRNAs) are a class of small RNA molecules, about 21 nucleotides (nt) in length, that regulate gene expression in a variety of manners, including translational repression, mRNA cleavage and deadenylation. In addition, the kit also can be used for isolating total RNA if messenger RNA or other large RNAs are of interest.
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Applicazioni
Caratteristiche e vantaggi
- Developed to enhance the efficiency of isolating microRNA and other small RNA molecules directly from a wide range of biological samples.
- Facilitates rapid and effective extraction and concentration of miRNA in 30 minutes for downstream applications.
- Capable of extracting miRNA with high purity with no detectable large RNA.
- Hazardous organic extractions are not involved.
Note legali
mirPremier is a registered trademark of Merck KGaA, Darmstadt, Germany
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Classi di pericolo
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2
Codice della classe di stoccaggio
10 - Combustible liquids
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I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
Extraction of Small RNA and qPCR Validation of miRNAs in Vigna mungo
Paul S, et al.
Bio-protocol, 5(5), e1417-e1417 (2015)
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Genome biology, 18(1), 105-105 (2017-06-16)
Post-transcriptional regulation of gene expression can be achieved through the control of mRNA stability, cytoplasmic compartmentalization, 3' UTR length and translational efficacy. Spermiogenesis, a process through which haploid male germ cells differentiate into spermatozoa, represents an ideal model for studying
Xiaoqiong Tang et al.
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LncRNA mortal obligate RNA transcript (MORT) is downregulated in different types of cancer, indicating its involvement in cancer biology. In this study, MORT and miRNA-16 were both downregulated in plasma of mantle cell lymphoma (MCL) patients than that in the
MicroRNAs control mRNA fate by compartmentalization based on 3? UTR length in male germ cells
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Genome Biology, 18(1), 105-105 (2017)
Identification of microRNA-mRNA regulatory network associated with oxidative DNA damage in human astrocytes
Nwokwu CD, et al.
ASN Neuro, 14, 17590914221101704-17590914221101704 (2022)
Contenuto correlato
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How can I avoid co-purifying mRNAs and rRNAs when purifying small RNAs from gram negative bacteria using mirPremier® microRNA Isolation Kit?
1 answer-
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Can I purify 18S and 28S large RNAs along with miRNAs with the mirPremier® microRNA Isolation Kit?
1 answer-
Large RNAs (18S and 28S) from the pellet fraction can be purified after transferring the microRNA-containing supernatant to a new tube by using the total RNA protocol or by phenol/chloroform extraction.
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Has the mirPremier® microRNA Isolation Kit been tested on sperm cells?
1 answer-
We have not tested mirPremier™ microRNA Isolation Kit with sperm cells.
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Can the mirPremier® microRNA Isolation Kit be used to isolate small RNAs from purified total RNA?
1 answer-
We have used to the kit to purify in vitro transcribed small RNAs with great success, but have not done so with purified total RNA. Here are the steps we would recommend trying with purified total RNA:1. Prepare a lysis mix with 0.7 vol. Small RNA Lysis Buffer (M1070) and 0.3 vol. Binding Solution (L8042).2. Add 500 ul of lysis mix with 50 ul total RNA and mix thoroughly.3. Spin at 14000 rpm for 5 min to precipitate large RNA.5. Transfer the supernatant to a new tube.6. Add 610 ul (1.1 vol.) 100% ethanol to the supernatant and mix well.7.Transfer the mixture to a binding column and spin 1 min to bind. Repeat the binding step with the remaining mixture.8. Wash the column first with 700 ul 100% ethanol, and then with ethanol-diluted wash Solution 2.9. Dry the column and elute small RNA.
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