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MilliporeSigma

S1452

MISSION® siRNA Transfection Reagent

Synonym(s):

MISSION® siRNA Transfection Reagent

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100 μL

$93.00

1 ML

$492.00

$93.00

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About This Item

NACRES:
NA.51
UNSPSC Code:
41106502

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product line

MISSION®

form

aqueous solution

shipped in

wet ice

storage temp.

2-8°C

Quality Level

200

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MISSION® siRNA Transfection Reagent

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X-tremeGENE™ siRNA Transfection Reagent Polymer reagent for delivering siRNA to common cell lines

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X-tremeGENE™ siRNA Transfection Reagent

product line

MISSION®

product line

-

product line

MISSION®

product line

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−70°C

storage temp.

2-8°C

form

aqueous solution

form

liquid

form

-

form

-

shipped in

wet ice

shipped in

wet ice

shipped in

dry ice

shipped in

wet ice

Quality Level

200

Quality Level

100

Quality Level

200

Quality Level

100

General description

The MISSION® siRNA Transfection Reagent is for the transfection of siRNA to achieve ≥90% silencing efficiency for transient knockdown of eukaryotic gene expression.
Effective gene silencing and reduced off target effects are achieved using a low siRNA concentration (1nM). The siRNA Transfection Reagent is provided as a sterile solution, and is compatible with serum and antibiotics.

Other Notes

Gene silencing assay: An A549 cell line stably expressing firefly luciferase was transfected in 24-well plates with 8.4 ng of 21-mer Luciferase siRNA using 2 μL of the MISSION® siRNA Transfection Reagent. Experiments were performed in triplicate in presence of serum. Cell lysates were assessed for luciferase expression 48 hours post-transfection.

Legal Information

MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

related product

Product No.
Description
Pricing

pictograms

Flame
GHS02

signalword

Warning

hcodes

H226

Hazard Classifications

Flam. Liq. 3

Storage Class

3 - Flammable liquids

wgk

WGK 3

flash_point_f

120.2 °F

flash_point_c

49 °C

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Certificates of Analysis (COA)

Lot/Batch Number
SLCQ2525
SLCL7789
SLCJ4575
SLCJ2572
SLCG8596

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Tuyen T Dang et al.
Cancers, 12(7) (2020-07-11)
It was recently shown that the 5' to 3' exoribonuclease XRN2 is involved in the DNA damage response. Importantly, loss of XRN2 abrogates DNA double stranded break repair via the non-homologous end-joining pathway. However, the mechanistic details of how XRN2
Ilaria Guerriero et al.
Oncotarget, 8(12), 19172-19191 (2016-11-24)
Hyperactivation of the PI3K/AKT pathway is observed in most human cancer including lung carcinomas. Here we have investigated the role of miRNAs as downstream targets of activated PI3K/AKT signaling in Non Small Cell Lung Cancer (NSCLC). To this aim, miRNA
A E Witt et al.
Oncogene, 36(12), 1707-1720 (2016-10-04)
Tumours are comprised of a highly heterogeneous population of cells, of which only a small subset of stem-like cells possess the ability to regenerate tumours in vivo. These cancer stem cells (CSCs) represent a significant clinical challenge as they are
Ponnuraj Nagendraprabhu et al.
PLoS pathogens, 13(12), e1006779-e1006779 (2017-12-16)
Poxviruses have evolved multiple strategies to subvert signaling by Nuclear Factor κB (NF-κB), a crucial regulator of host innate immune responses. Here, we describe an orf virus (ORFV) virion-associated protein, ORFV119, which inhibits NF-κB signaling very early in infection (≤
Tuyen T Dang et al.
International journal of molecular sciences, 21(12) (2020-06-19)
Cellular survival is dependent on the efficient replication and transmission of genomic information. DNA damage can be introduced into the genome by several different methods, one being the act of DNA replication. Replication is a potent source of DNA damage
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