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SHC016V

Sigma-Aldrich

MISSION® pLKO.1-puro Non-Target shRNA Control Transduction Particles

Targets no known genes from any species

Sinonimo/i:

MISSION®, MISSION® Control Transduction Particles, negative control, negative shRNA control, non-target control, non-target shRNA, non-target shRNA control, shRNA control

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About This Item

Codice UNSPSC:
41106609
NACRES:
NA.51

Livello qualitativo

100

Nome Commerciale

MISSION®

Concentrazione

≥1x106 VP/ml (via p24 assay)

Condizioni di spedizione

dry ice

Temperatura di conservazione

−70°C

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Categorie correlate

Descrizione generale

The MISSION® pLKO.1-puro Non-Target shRNA Control Transduction Particles contain an shRNA insert that does not target any known genes from any species, making it useful as a negative control in experiments using the MISSION® shRNA library clones. This allows one to examine the effect of transduction of a short-hairpin on gene expression and interpret the knockdown effect seen with shRNA clones. Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. The Non-Target shRNA Control Transduction Particles are provided as 200 μL at 1 x 106 TU/mL via p24 assay.
When conducting experiments using MISSION® shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results. The MISSION Control Transduction Particles are a critical positive control to monitor transduction efficiency.
To see more application data, protocols, vector maps visit sigma.com/shrna.

Applicazioni

MISSION® pLKO.1-puro Non-Target shRNA Control Transduction Particles have been used to generate 3T3-L1 (pre-adipocytes) control cell lines.

Risultati analitici

To see more application data, protocols, vector maps visit sigma.com/shrna.

Note legali

MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

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Raccomandato

N° Catalogo
Descrizione
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SHC001V

MISSION® pLKO.1-puro Empty Vector Control Transduction Particles, Contains no shRNA insert

SHC002V

MISSION® pLKO.1-puro Non-Mammalian shRNA Control Transduction Particles, Targets no known mammalian genes

SHC003V

MISSION® pLKO.1-puro-CMV-TurboGFP™ Positive Control Transduction Particles, Green fluorescent protein marker to monitor transduction efficiency

SHC007V

MISSION® Luciferase shRNA Control Transduction Particles, shRNA sequence targeting luciferase

SHC012V

MISSION® pLKO.1-puro-CMV-TagRFP™ Positive Control Transduction Particles, Contains a gene encoding TagRFP

SHC014V

MISSION® pLKO.1-puro-UbC-TurboGFP™ Positive Control Transduction Particles, Contains a gene encoding TurboGFP, under the control of the UbC promoter

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

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Certificati d'analisi (COA)

Lot/Batch Number
01212208MN
03162006MN
07181827MN

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