SHC005V
MISSION® eGFP Control Transduction Particles
shRNA sequence targeting eGFP
Sinonimo/i:
MISSION®, MISSION® Control Transduction Particles
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Informazioni su questo articolo
Quality Segment
product line
MISSION®
concentration
≥1x106 VP/ml (via p24 assay)
technique(s)
capture ELISA: 106 TU/mL using p24
shipped in
dry ice
storage temp.
−70°C
General description
To see more application data, protocols, vector maps visit sigma.com/shrna.
The eGFP shRNA Control Transduction Particles are produced from the sequence-verified lentiviral plasmid, pLKO.1-puro-eGFP shRNA (Prod. No. SHC005). Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. In addition, the control transduction particles are pseudotyped with an envelope G glycoprotein from Vesicular Stomatitis Virus (VSV-G), allowing transduction of a wide variety of mammalian cells. 200 μl of 106 TU/ml (via p24 titering assay) lentiviral particles are provided as frozen stock.
Application
Legal Information
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| description shRNA sequence targeting eGFP | description Contains no shRNA insert | description TurboGFP™, High Titer, Green fluorescent protein marker to monitor transduction efficiency | description Contains no shRNA insert |
| concentration ≥1x106 VP/ml (via p24 assay) | concentration ≥1x106 VP/ml (via p24 assay) | concentration ≥1x109 VP/ml (via p24 assay) | concentration ≥1x109 VP/ml (via p24 assay) |
| product line MISSION® | product line MISSION® | product line MISSION® | product line MISSION® |
| technique(s) capture ELISA: 106 TU/mL using p24 | technique(s) capture ELISA: 106 TU/mL using p24 | technique(s) capture ELISA: 109 TU/mL using p24 | technique(s) capture ELISA: 109 TU/mL using p24 |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
| storage temp. −70°C | storage temp. −70°C | storage temp. −70°C | storage temp. −70°C |
| Quality Level 200 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
Classe di stoccaggio
12 - Non Combustible Liquids
Che succede?
WGK 3
Punto di infiammabilità (°F)
Not applicable
Punto di infiammabilità (°C)
Not applicable
DPI (dispositivi di protezione individuale)
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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