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S6311

Sigma-Aldrich

Anti-phospho-S6-Kinase (pThr389) antibody produced in rabbit

affinity isolated antibody, buffered aqueous glycerol solution

Sinonimo/i:

Anti-phospho-p70S6K (pThr389)

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About This Item

Numero MDL:
MFCD02097170
Codice UNSPSC:
12352203
NACRES:
NA.44

Origine biologica

rabbit

Livello qualitativo

200

Coniugato

unconjugated

Forma dell’anticorpo

affinity isolated antibody

Tipo di anticorpo

primary antibodies

Clone

polyclonal

Forma fisica

buffered aqueous glycerol solution

Reattività contro le specie

mouse, rat, human

tecniche

western blot (chemiluminescent): 1:1,000 using serum-treated NIH3T3 cell extract, or insulin-treated HeLa, COS, and C6 cell extracts

N° accesso UniProt

P23443

Condizioni di spedizione

wet ice

Temperatura di conservazione

−20°C

Informazioni sul gene

human ... RPS6KB1(6198)
mouse ... Rps6kb1(72508)
rat ... Rps6kb1(83840)

Descrizione generale

p70 S6 kinase or p70S6K is a protein that belongs to Ser/Thr protein kinase family. It has a crucial role in cell growth and G1 cell cycle progression. It also has a pivotal role in phosphorylating eEF2k at a conserved serine residue and hence regulating the protein synthesis. Rabbit anti-phospho-(p70S6K) antibody can identify p70 S6 kinase and p85 S6 kinase when phosphorylated at (Thr389). Anti-phospho-S6-kinase (pThr389) antibody reacts specifically with p70 S6 kinase of rat, mouse and human. This product can also detect few phosphorylated isoforms of Protein Kinase C when present at high level in cells.

Immunogeno

A synthetic phospho-(Thr389) peptide corresponding to residues around (Thr389) of human p70 S6 kinase, coupled to KLH.

Applicazioni

Anti-phospho-S6-kinase (pThr389) antibody can be used in western blotting (chemiluminescent) (diluted 1:1000) using serum-treated NIH3T3 cell extract or insulin-treated HeLa, COS, and C6 cell extracts.

Stato fisico

Solution in 10 mM sodium HEPES, pH 7.5, containing 150 mM sodium chloride, 100 μg/ml bovine serum albumin and 50% glycerol

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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J Chung et al.
Nature, 370(6484), 71-75 (1994-07-07)
Platelet-derived growth factor receptor (PDGF-R) phosphorylation at tyrosines 740/751 and insulin receptor phosphorylation of insulin receptor substrate-1 effects the recruitment and activation of phosphatidylinositol-3-OH kinase (PI(3)K). Changes in PI(3)K activity correlate with cell growth but its downstream signal transducers are
N Pullen et al.
FEBS letters, 410(1), 78-82 (1997-06-23)
The activation of p70s6k is accompanied by a complex series of phosphorylation events. In this review we propose a model of activation which divides p70s6k into four functional modules that cooperate in leading to full enzyme activity. In the light
Diane Evrard et al.
Cancers, 14(18) (2022-09-24)
Mammalian target of rapamycin (mTOR) regulates cellular functions by integrating intracellular signals and signals from the tumor microenvironment (TME). The PI3K-AKT-mTOR pathway is activated in 70% of head and neck squamous cell carcinoma (HNSCC) and associated with poor prognosis. This
X Wang et al.
The EMBO journal, 20(16), 4370-4379 (2001-08-14)
Elongation factor 2 kinase (eEF2k) phosphorylates and inactivates eEF2. Insulin induces dephosphorylation of eEF2 and inactivation of eEF2 kinase, and these effects are blocked by rapamycin, which inhibits the mammalian target of rapamycin, mTOR. However, the signalling mechanisms underlying these
James Yu et al.
PloS one, 6(7), e21415-e21415 (2011-07-14)
We have shown that inhibition of mTOR in granulosa cells and ovarian follicles results in compromised granulosa proliferation and reduced follicle growth. Further analysis here using spontaneously immortalized rat granulosa cells has revealed that mTOR pathway activity is enhanced during
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