S4920
SITE Liquid Media Supplement (100×)
liquid, sterile-filtered, BioReagent, suitable for cell culture
Sinonimo/i:
Media supplement
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Cambia visualizzazione
5 ML
CHF 103.00
About This Item
Codice UNSPSC:
12352205
NACRES:
NA.75
Prodotti consigliati
Sterilità
sterile-filtered
Nome Commerciale
BioReagent
Stato
liquid
tecniche
cell culture | mammalian: suitable
Impurezze
endotoxin, tested
Condizioni di spedizione
ambient
Temperatura di conservazione
2-8°C
Descrizione generale
SITE Liquid Media Supplement (100×) serves as a substitute for serum-free formulations. It contains purified factors required for in vitro growth and differentiation studies. The addition of supplements to media will vary, depending on the cell type being studied and the basal medium employed. It is a general cell supplement designed for use in non-complex media (e.g., minimum essential medium (MEM), Roswell Park Memorial Institute Medium (RPMI-1640)) and complex media (e.g., Ham′s F-12, Dulbecco′s Modified Eagle Medium (DME) /F-12, MEM) with sodium pyruvate.
Applicazioni
Azioni biochim/fisiol
- Insulin: a polypeptide hormone that promotes the uptake of glucose and amino acids and may owe an observed mitogenic effect to this property.[4]
- Transferrin: an iron-transport protein. Iron is an essential trace element but can be toxic in its free form. To nourish cells in culture, iron is supplied bound to transferrin in serum.[5]
- Selenium: an essential trace element normally provided by serum.[6]
- Ethanolamine: plays a significant role in the proliferation of hybridoma cells and frequently is added to supplements used for culturing these cells.[7]
Componenti
Contains 1.0mg/ml recombinant human insulin, 0.55mg/ml human transferrin (substantially iron-free), 0.5μg/ml sodium selenite and 0.2mg/ml ethanolamine at the 100x concentration.
Nota sulla preparazione
Prepared in Earle′s Balanced Salt Solution (EBSS) without phenol red.
Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
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I clienti hanno visto anche
H Murakami et al.
Proceedings of the National Academy of Sciences of the United States of America, 79(4), 1158-1162 (1982-02-01)
A serum-free medium supplemented with a few growth factors was devised to grow lymphocyte hybridomas. The medium was developed with the hybridoma line MPC11-BL, a fusion product between a mouse plasmacytoma cell line (MPC11TG70na3) and mouse (BALB/c) spleen cells. In
Lixin Zhu et al.
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In a comparison of three different tissues, the membrane cytoskeleton linker protein ezrin was found to assume high levels of phosphorylation on threonine-567 (T567) in the brush border membranes of renal proximal tubule cells and small intestine enterocytes, in contrast
Rawan Eid et al.
Biochimica et biophysica acta. Molecular cell research, 1864(2), 399-430 (2016-12-13)
Iron is an essential micronutrient that is problematic for biological systems since it is toxic as it generates free radicals by interconverting between ferrous (Fe2+) and ferric (Fe3+) forms. Additionally, even though iron is abundant, it is largely insoluble so
Lixin Zhu et al.
American journal of physiology. Cell physiology, 293(3), C874-C884 (2007-06-08)
In its dormant state, the membrane cytoskeletal linker protein ezrin takes on a NH(2) terminal-to-COOH terminal (N-C) binding conformation. In vitro evidence suggests that eliminating the N-C binding conformation by Thr(567) phosphorylation leads to ezrin activation. Here, we found for
Nico Laur et al.
PloS one, 15(5), e0233357-e0233357 (2020-05-21)
Trace elements and minerals are compounds that are essential for the support of a variety of biological functions and play an important role in the formation of and the defense against oxidative stress. Here we describe a technique, allowing sequential
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