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S2014

Sigma-Aldrich

Staphylococcus aureus

buffered aqueous suspension, Wood 46 strain

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About This Item

Codice UNSPSC:
12352202
NACRES:
NA.81
Prezzi e disponibilità al momento non sono disponibili

Origine biologica

Staphylococcus aureus

Sterilità

Not processed or packaged aseptically

Stato

buffered aqueous suspension

Composizione

Cell suspension, ~10% wet weight/volume

Temperatura di conservazione

2-8°C

Descrizione generale

Staphylococcus aureus Wood 46 is protein A deficient and spa negative.[1] It shares 98% to 99% genome identity with S.aureus[1] and shows a lower surface expression of cell wall-associated protein A.[2]

Applicazioni

(Not intended for use as a starter culture.)
Staphylococcus aureus has been used:
  • to mimic infection and induce fever in Pekin duck[3]
  • to test its effect on hemocyte morphology in hemolymph samples from beetle Tenebrio molitor larva[4]
  • in the antibacterial activity and minimum inhibitory concentration (MIC) assay with gedunin and 7-deacetoxy-7αhydroxygedunin potassium salt [5]

Wood 46, a non-protein A producing S. aureus strain[6], prepared by the same method as P7155 (Protein A, crude cell suspension-Cowan strain), may be used as a control in protein A-immunoglobulin binding studies.

Azioni biochim/fisiol

Staphylococcus aureus Wood 46 displays reduced virulence compared to the S. aureus.[1] This isolate is useful in understanding protein A role in pathogenesis and virulence.[2]

Stato fisico

Formalin-fixed crude cell suspension of essentially non-viable S. aureus (Wood 46 strain) in 0.05 M potassium phosphate buffer, pH 7.5, containing 0.2% sodium azide

Nota sulla preparazione

Produced in pure culture.

Risultati analitici

This strain binds less than 10% of the rabbit IgG bound by P 7155 as assayed by a modification of the method of Kessler[7].

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


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Synthetic modification of gedunin and comparative antibacterial activity of gedunin and 7-deacetoxy-7alpha-hydroxygedunin potassium salt
Okhale SE, et al.
African Journal of Pure and Applied Chemistry, 6(14), 183-189 (2012)
S W Kessler
Journal of immunology (Baltimore, Md. : 1950), 117(5 Pt 1), 1482-1490 (1976-11-01)
Procedures are detailed for the rapid isolation of representative cell membrane antigens with protein A-bearing staphylococci as an adsorbent for IgG antibodies complexed with the antigens. Cell surface membrane proteins were radioiodinated and solubilized in nonionic detergent. Specific antisera were
G Kronvall et al.
Infection and immunity, 3(1), 10-15 (1971-01-01)
Protein A of Staphylococcus aureus can be detected on cell walls of intact bacteria by use of radioactively labeled myeloma globulin. Of 156 strains of S. aureus, 141 (90%) contained protein A. None of 47 S. epidermidis strains was positive
Manasi Balachandran et al.
Genome announcements, 5(13) (2017-04-01)
Here, we report the first complete genome sequence of the Staphylococcus aureus strain Wood 46. Wood 46 has played an important role in understanding the virulence and pathogenesis of S. aureus infections. This report will assist efforts in vaccine development against
Arkadiusz Urbański et al.
Scientific reports, 12(1), 17277-17277 (2022-10-15)
Tachykinins (TKs) are a group of conserved neuropeptides. In insects, tachykinin-related peptides (TRPs) are important modulators of several functions such as nociception and lipid metabolism. Recently, it has become clear that TRPs also play a role in regulating the insect

Questions

  1. Hello, I'm wondering if this "Staphylococcus aureus, wood 46 strain" is deactivated or not. Additionally, do you have the corresponding aptamer or antibody of this strain?

    1 answer
    1. This product is a formalin-fixed crude cell suspension of essentially non-viable S. aureus in 0.05 M potassium phosphate buffer containing 0.2% sodium azide. Currently, there is no corresponding aptamer or antibody for the Wood 46 Strain.

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