RAB0275

Mouse IL-1 β ELISA Kit

for cell and tissue lysates

• Sinonimo/i: Cytokine detection kit
• Codice UNSPSC: 41116158
• NACRES: NA.32

Proprietà

• Reattività contro le specie: mouse
• Confezionamento: kit of 96 wells (12 strips x 8 wells)
• tecniche: ELISA: suitable; capture ELISA: suitable
• input: sample type cell lysate; sample type tissue lysate
• assay range: inter-assay cv: <12%; intra-assay cv: <10%; sensitivity: 5 pg/mL; standard curve range: 2.74-2000 pg/mL
• Metodo di rivelazione: colorimetric
• Condizioni di spedizione: wet ice
• Temperatura di conservazione: −20°C
• Informazioni sul gene: mouse ... Il1b(16176)

Descrizione

Descrizione generale

The Mouse IL-1 β ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IL-1 β cell lysate and tissue lysate.

Immunogeno

Recombinant Mouse IL-1 β

Applicazioni

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Mouse IL-1 β ELISA Kit has been used to measure the concentration of interleukin 1 (IL)1-β in serum[1] and bronchoalveolar lavage fluid (BALF) sample.[2]

Azioni biochim/fisiol

Interleukin 1 β (IL-1β) stimulates the production of endogenous 92 kDa gelatinase (matrix metalloproteinases 9 (MMP-9)) and tissue inhibitors of MMP (TIMP -1).[3] It is a proinflammatory cytokine that induces various acute and chronic inflammation. Therefore, IL-1β can be considered as a potential therapeutic target for disease associated with fibrosis and tissue remodeling.[4] IL-1β brings neutrophil and macrophage to the site of infection.[5] Over expression of IL-1β in synovial fluid contributes to the pathogenesis of synovitis and degenerative changes of the cartilaginous tissue and bone of the temporomandibular joint.[6] Mutations in IL-1β gene leads to renal manifestations and renal sequelae in Henoch-Schonlein purpura (HSP).[7]

Altre note

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

I componenti del kit sono disponibili anche separatamente

• RABDIL6 ELISA 5X Sample Diluent Buffer (Item D2)
• RABELADE ELISA 5X Assay/Sample Diluent Buffer E (Item E2)
• RABLYSIS1 2X Cell Lysis Buffer (Item J)
• RABSTOP3 ELISA Stop Solution (Item I)
• RABTMB3 ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)
• RABWASH4 20X Wash Buffer (Item B)

Informazioni sulla sicurezza

• Pittogrammi: GHS05
• Avvertenze: Warning
• Indicazioni di pericolo: H290
• Consigli di prudenza: P234 - P390
• Classi di pericolo: Met. Corr. 1
• Codice della classe di stoccaggio: 8A - Combustible corrosive hazardous materials