RAB0274

Mouse IL-1 β ELISA Kit

for serum, plasma and cell culture supernatant

• Sinonimo/i: Il-1 beta, Interleukin-1 beta
• Codice UNSPSC: 41116158
• NACRES: NA.32

Proprietà

• Reattività contro le specie: mouse
• Confezionamento: kit of 96 wells (12 strips x 8 wells)
• tecniche: ELISA: suitable; capture ELISA: suitable
• input: sample type plasma; sample type serum; sample type cell culture supernatant(s)
• assay range: inter-assay cv: <12%; intra-assay cv: <10%; sensitivity: 5 pg/mL; standard curve range: 2.74-2000 pg/mL
• Metodo di rivelazione: colorimetric
• Condizioni di spedizione: wet ice
• Temperatura di conservazione: −20°C
• Informazioni sul gene: mouse ... Il1b(16176)

Descrizione

Descrizione generale

The Mouse IL-1β ELISA (enzyme-linked immunosorbent assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IL-1β in serum, plasma, cell culture supernatants and urine. Interleukin-1β (IL-1β) cytokine is generated by monocytes, dendritic cells, macrophages and keratinocytes. Pro-IL-1β, an IL-1β precursor undergoes proteolytic cleavage to produce its active form of 17 kDa.[1] IL-1β is a potent inflammatory cytokine,[1] and mediates inflammation through proinflammatory molecules: cyclooxygenase-2 (COX2) and nitric oxide.[2] IL-1β release is stimulated by injury and increased levels are observed in response to severe pain.[3] It has its main function in T-cell activation, antigen recognition and T-helper cell 17 cell development. IL-1β is also responsible for the formation of IL-17 and IL-22 by nature killer T (NKT) cells and NK cells. It is known to induce inflammation in psoriasis and rheumatoid arthritis.[1] IL-1β controls intercellular adhesion molecule-1 expression. It is significantly associated with bone damage and vascular injury.[4]

Immunogeno

Recombinant Mouse IL-1 β

Applicazioni

Mouse IL-1 β ELISA Kit has been used to determine the presence of interleukin-1β (IL-1β) in plasma sample.[5] It has also been used to determine the serum concentration levels of IL-1β.[6][7]

Altre note

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

I componenti del kit sono disponibili anche separatamente

• RABELADA ELISA 1X Assay/Sample Diluent Buffer A (Item D1)
• RABELADB ELISA 5X Assay/Sample Diluent Buffer B (Item E1)
• RABSTOP3 ELISA Stop Solution (Item I)
• RABTMB3 ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)
• RABWASH4 20X Wash Buffer (Item B)

Informazioni sulla sicurezza

• Pittogrammi: GHS05
• Avvertenze: Warning
• Indicazioni di pericolo: H290
• Consigli di prudenza: P234 - P390
• Classi di pericolo: Met. Corr. 1
• Codice della classe di stoccaggio: 8A - Combustible corrosive hazardous materials