R1137
Hind III from Haemophilus influenzae
Restriction Enzyme
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About This Item
Numero CAS:
Numero MDL:
Codice UNSPSC:
12352204
Prodotti consigliati
Grado
for molecular biology
Stato
buffered aqueous glycerol solution
Concentrazione
≥10000 units/mL
10,000 units/mL
Condizioni di spedizione
wet ice
Temperatura di conservazione
−20°C
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Specificità
Recognition sequence: 5′-A/AGCTT-3′
Cutting results: 2-10-fold Hind III overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: 65 °C for 15 minutes
Cutting results: 2-10-fold Hind III overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: 65 °C for 15 minutes
Applicazioni
HindIII, a restriction endonuclease, is used in molecular biology applications to cleave DNA at the recognition site 5′-A/AGCTT-3′ to generate DNA fragments with cohesive 5′-ends.
Altre note
Supplied with 10x Restriction Enzyme Buffer SB (B8781)
Comment: Hind III under suboptimal reaction conditions will cleave secondary recognition sites (star activity).
Stato fisico
Solution in 10 mM Tris-HCl, pH 7.5, 0.1 mM EDTA, 1 mM dithioerythritol, 250 mM NaCl, 0.01% polydocanol (v/v), 50% glycerol (v/v) at 4 °C
Prodotti correlati
N° Catalogo
Descrizione
Determinazione del prezzo
Tampone di incubazione
N° Catalogo
Descrizione
Determinazione del prezzo
Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
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