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PP2386

Sigma-Aldrich

Yeast Tag Vector Set

plasmid vectors for molecular cloning

Sinonimo/i:

cloning vector, expression vector, molecular cloning vector, plasmid, plasmid vector, snapfast vector, vector

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About This Item

Codice UNSPSC:
12352200

Coniugato

E-Tag
S-Tag
streptavidin conjugate

Tag

10-His tagged
FLAG® tagged
GST tagged
Glu-Glu tagged
Hemagglutinin A (HA) tagged
Herpes Simplex Virus (HSV) tagged
T7 tagged
V5 tagged
c-Myc tagged
maltose-binding protein (MBP) tagged

Stato

buffered aqueous solution

Selezione batterica

kanamycin

Origine di replicazione

2Micron
pUC (500 copies)

Clivaggio proteico

no cleavage

Posizione del tag peptidico

N-terminal

Promotore

Promoter name: TEF1
Promoter activity: constitutive
Promoter type: yeast

Condizioni di spedizione

ambient

Temperatura di conservazione

−20°C

Descrizione generale

Molecular cloning often benefits from optimizing the vector used for expression.

This pack provides our entire range of 10 yeast functional tags, including epitope, affinity and solubility tags. Inserting your gene of interest into the MCS will place the tag downstream (at the C terminus of the protein), and the gene will be regulated by the strong constitutive yeast TEF1 promoter. This pack therefore provides a versatile resource for adding functional tags to several plasmids, but also enables you to compare different tags on the same protein, to identify which works best in your experimental system.This plasmid set has been designed to be compatible with a range of cloning techniques. The multiple cloning site contains a range of standard commonly used restriction sites for cloning. Using these sites genes can be inserted using standard cloning methods with DNA ligase. Other methods such as ligase independent cloning (LIC) Gibson Assembly InFusionHD or Seamless GeneArt can also be used and because all of our plasmids are based on the same backbone the same method can be used for cloning into all of our catalogue vectors.

Multiple cloning site notes: There are a few important sites within the MCS. These include the NcoI site the XbaI site and the BsgI and BseRI sites. The NcoI site contains a start codon that is immediately downstream of both a Kozak and Shine-Dalgarno ribosomal binding site. These allow for optimal positioning of genes when the start codon is placed in this location. If this is not required and you wish to use a downstream site for gene cloning you can remove the NcoI site by cleaving the plasmid with KpnI. The XbaI site contains a stop codon. This stop codon is positioned in a specific position in relation to the BsgI and BseRI sites that are immediately downstream. When either BseRI or BsgI cleave the plasmid they produce a TA overhang from the stop codon in the XbaI site that is compatible with all of our peptide tag plasmids cut with the same sites. BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding site. Whenever we clone a gene into our multiple cloning site we always position the start and stop codon in the same positions in the MCS. If the start and ends of the genes are not compatible with NcoI and XbaI we extend the sequence to the nearest external sites but keep the start and stop codons locations consistent.

Transcription Termination: These plasmids contains three alternative transcription terminators for mammalian bacterial and bacteriophage (T7) expression. This means that only the promoter needs to be changed to alter the expression system you are using. We sell multiple promoters that can be used in each of these systems. The presence of each terminator does not reduce expression in the alternative systems.

Sequenza

For Genebank sequence, FASTA sequence, Quick-reference Plasmid Map, and Full Plasmid Map, see the individual vector product page for links.

Risultati analitici

To view the Certificate of Analysis for this product, please visit www.oxfordgenetics.com.

Altre note

Looking for more vector options to move your experiments forward faster? Consider a custom cloning vector designed and built by Oxford Genetics. Find out more at Oxford Genetics - Sigma's partner for cloning and expression vectors for molecular biology and synthetic biology applications.

Note legali

These plasmids are sold free of reach-through rights and can be used to make commercial products. However the plasmids themselves (or derivatives) cannot be sold.
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Oxford Genetics is a trademark of Oxford Genetics Ltd

I componenti del kit sono disponibili anche separatamente

N° Catalogo
Descrizione
SDS

  • OGS3456PSF-TEF1-COOH-HIS6 - C-TERMINAL 6 HIS TAG YEAST PLASMID, plasmid vector for molecular cloningSDS

  • OGS3458PSF-TEF1-COOH-FLAG® - C-TERMINAL FLAG® TAG YEAST PLASMID, plasmid vector for molecular cloningSDS

  • OGS3459PSF-TEF1-COOH-CMYC - C-TERMINAL C-MYC TAG YEAST PLASMID, plasmid vector for molecular cloningSDS

  • PSF-TEF1-COOH-HA - C-TERMINAL HA TAG YEAST PLASMID, plasmid vector for molecular cloning

  • PSF-TEF1-COOH-GST - C-TERMINAL GST TAG YEAST PLASMID, plasmid vector for molecular cloning

  • PSF-TEF1-COOH-MBP - C-TERMINAL MBP TAG YEAST PLASMID, plasmid vector for molecular cloning

Prodotti correlati

N° Catalogo
Descrizione
Determinazione del prezzo

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


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