P7275
Pyrophosphate reagent
spectrophotometric assay
Sinonimo/i:
Pyrophosphate detection reagent
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1 VIAL
CHF 82.20
10 VIALS
CHF 428.00
CHF 82.20
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Cambia visualizzazione
1 VIAL
CHF 82.20
10 VIALS
CHF 428.00
About This Item
Codice UNSPSC:
12352204
NACRES:
NA.54
CHF 82.20
Check Cart for Availability
Prodotti consigliati
Livello qualitativo
Stato
powder
impiego
vial sufficient for ≤4 assays
Temperatura di conservazione
−20°C
Categorie correlate
Applicazioni
Azioni biochim/fisiol
The pyrophosphate detection kit couples the production of pyrophosphate to the consumption of NADH[3].
Confezionamento
A technical bulletin is included.
Componenti
ComponentConcentration
Imidazole × HCl, pH 7.4 45 mM
Citrate 5 mM
EDTA 0.10 mM
Mg2+, Mn2+, Co2+ 2 mM, 0.2 mM, 0.02 mM
β-NADH 0.8 mM
F-6-P 12 mM
Bovine Serum Albumin 5 mg/mL
Sugar Stabilizer 5 mg/mL
PPi-PFK 0.5 units/mL
Aldolase 7.5 units/mL
GDH 5 units/mL
TPI 50 units/mL
Imidazole × HCl, pH 7.4 45 mM
Citrate 5 mM
EDTA 0.10 mM
Mg2+, Mn2+, Co2+ 2 mM, 0.2 mM, 0.02 mM
β-NADH 0.8 mM
F-6-P 12 mM
Bovine Serum Albumin 5 mg/mL
Sugar Stabilizer 5 mg/mL
PPi-PFK 0.5 units/mL
Aldolase 7.5 units/mL
GDH 5 units/mL
TPI 50 units/mL
Qualità
A coupled enzyme system containing fructose-6-phosphate kinase (pyrophosphate-dependent), aldolase, triosephosphate isomerase, and α-glycerophosphate dehydrogenase.
Principio
Two moles of NADH are oxidized to NAD+ per mole of pyrophosphate consumed. The reaction is monitored spectrophotometrically at 340 nm.
The reagent can be used to detect 10-100 nmoles of pyrophosphate.
The reagent can be used to detect 10-100 nmoles of pyrophosphate.
Nota sulla preparazione
Dissolve the contents of one vial of Pyrophosphate Reagent in 4.0 mL of water.
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Gloves, type N95 (US)
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