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P4524

Sigma-Aldrich

β-Lactamase from Enterobacter cloacae

Type IV, lyophilized powder, 0.2-0.6 units/mg protein (using benzylpenicillin)

Sinonimo/i:

β-Lactamase I, β-Lactamase II, Cephalosporinase, Penicillin amido-β-lactam hydrolase, Penicillinase from Enterobacter cloacae

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About This Item

Numero CAS:
9073-60-3
Classificazione EC (Enzyme Commission):
3.5.2.6 (BRENDA, IUBMB)
Numero MDL:
MFCD00131819
Codice UNSPSC:
12352204
NACRES:
NA.54

Origine biologica

bacterial (Enterobacter cloacae)

Livello qualitativo

200

Tipo

Type IV

Forma fisica

lyophilized powder

Attività specifica

0.2-0.6 units/mg protein (using benzylpenicillin)

PM

29 kDa

Composizione

Protein, ~60%

tecniche

enzyme immunoassay: suitable

Compatibilità

suitable for enzyme test

applicazioni

pharmaceutical

Temperatura di conservazione

2-8°C

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Descrizione generale

β-Lactamase is a monomeric enzyme of 29 kDa. This product is produced from Enterobacter cloacae and is provided as a lyophilized powder. It acts as a target protein for β-lactam antibiotics. This enzyme is present in Gram-negative bacteria.

Applicazioni

β--lactamase is used to inactivate β-lactam antibiotics by breaking open the β-lactam ring. β--lactamase is used to study antibiotic resistance and resistance suppression. Product P4524 is produced from Enterobacter cloacae and is provided as a lyophilized powder.
β-Lactamase from Enterobacter cloacae has been used in the maternal and fetal quantitative blood cultures to avoid any carryover phenomenon. It has also been used as a component in Mueller Hinton (MH) broth for placental cultures.

Azioni biochim/fisiol

β--lactamase inactivates β-lactam antibiotics by breaking open the β-lactam ring.

Definizione di unità

One unit will hydrolyze 1.0 μmole of benzylpenicillin per min at pH 7.0 at 25 °C. This International Unit (using benzylpenicillin as substrate) is approximately equal to 600 Levy or 75 Pollock units.

Stato fisico

Lyophilized powder containing sodium phosphate buffer salts

Risultati analitici

Protein determined by biuret.

Inibitore

N° Catalogo
Descrizione
Determinazione del prezzo

Pittogrammi

Health hazard
GHS08

Avvertenze

Danger

Indicazioni di pericolo

H317,H334

Classi di pericolo

Resp. Sens. 1 - Skin Sens. 1

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Nitrocefin A chromogenic β-lactamase substrate that undergoes distinctive color change from yellow as the amide bond in the β-lactam ring is hydrolyzed by β-lactamase.

Sigma-Aldrich

484400

Nitrocefin

Cartap hydrochloride PESTANAL®, analytical standard

Supelco

45995

Cartap hydrochloride

Buprofezin PESTANAL®, analytical standard

Supelco

37886

Buprofezin

Persistent bacteremia in rabbit fetuses despite maternal antibiotic therapy in a novel intrauterine-infection model
Gras-Le Guen C, et al.
Antimicrobial Agents and Chemotherapy, 47(7), 2125-2130 (2003)
Protein-protein interactions monitored in mammalian cells via complementation of beta-lactamase enzyme fragments
Wehrman T, et al.
Proceedings of the National Academy of Sciences of the USA, 99(6), 3469-3474 (2002)
Past and present perspectives on beta$-lactamases
Bush K
Antimicrobial Agents and Chemotherapy, 62(10) (2018)
P J Johnsen et al.
Genetics, 181(4), 1521-1533 (2009-02-05)
We present a new hypothesis for the selective pressures responsible for maintaining natural competence and transformation. Our hypothesis is based in part on the observation that in Bacillus subtilis, where transformation is widespread, competence is associated with periods of nongrowth
Jung-Ho Shin et al.
EMBO reports, 22(2), e51790-e51790 (2021-01-20)
Bactericidal antibiotics are powerful agents due to their ability to convert essential bacterial functions into lethal processes. However, many important bacterial pathogens are remarkably tolerant against bactericidal antibiotics due to inducible damage repair responses. The cell wall damage response two-component
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