O3131
Oligo(dT)-Cellulose
For polyadenylated mRNA purification
Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali
About This Item
Numero MDL:
Codice UNSPSC:
12352200
Prodotti consigliati
Attività estranea
RNase, none detected
Temperatura di conservazione
−20°C
Cerchi prodotti simili? Visita Guida al confronto tra prodotti
Applicazioni
For polyadenylated mRNA purification using rapid and simple chromatographic techniques. Covalently attached oligothymidylic acid chains are approx. 20 nucleotides long. Eukaryotic mRNA specifically binds by its 3′-poly(A) tail. Non-polyadenylated RNA and other contaminants can be removed by washing, and the purified mRNA is eluted with a low salt buffer.
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Gloves, type N95 (US)
Scegli una delle versioni più recenti:
Possiedi già questo prodotto?
I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
Nikolay E Shirokikh et al.
Proceedings of the National Academy of Sciences of the United States of America, 105(31), 10738-10743 (2008-07-29)
Eukaryotic mRNAs in which a poly(A) sequence precedes the initiation codon are known to exhibit a significantly enhanced cap-independent translation, both in vivo and in cell-free translation systems. Consistent with high expression levels of poxviral mRNAs, they contain poly(A) sequences
A Krystosek et al.
The Journal of biological chemistry, 250(15), 6077-6084 (1975-08-10)
The polyadenylic acid-containing messenger ribonucleic acids of eukaryotic cells are rapidly isolated and deproteinized in a simple and gentle one-step procedure. The polyribosome fraction, dissolved in 0.5 M NaCl/0.5 percent sodium dodecyl sulfate, is passed through an oligo(dT)-cellulose column which
H Aviv et al.
Proceedings of the National Academy of Sciences of the United States of America, 69(6), 1408-1412 (1972-06-01)
A convenient technique for the partial purification of large quantities of functional, poly(adenylic acid)-rich mRNA is described. The method depends upon annealing poly(adenylic acid)-rich mRNA to oligothymidylic acid-cellulose columns and its elution with buffers of low ionic strength. Biologically active
Gilham
Journal of the American Chemical Society, 86, 4982-4982 (1964)
Peter G Fuerst et al.
Neuron, 64(4), 484-497 (2009-12-01)
DSCAM and DSCAM-LIKE1 (DSCAML1) serve diverse neurodevelopmental functions, including axon guidance, synaptic adhesion, and self-avoidance, depending on the species, cell type, and gene family member studied. We examined the function of DSCAM and DSCAML1 in the developing mouse retina. In
Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..
Contatta l'Assistenza Tecnica.