There are no definitive references or citations confirming the light sensitivity of this material. However, this product is packaged in amber glass may be susceptible to light, particularly at UV wavelengths. In bright field microscopy slides must be viewed in subdued light. While this item is not tested for use in fluorescent, it has been used on Flow Cytometry and has an emission wavelength of approximately 600 nm. There are a number of publication indicating light sensitivity. See the link below to review on such example:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5931440/
O1391
Oil Red O solution
0.5% in isopropanol
Sinonimo/i:
Solvent Red 27, Sudan Red 5B
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About This Item
Prezzi e disponibilità al momento non sono disponibili
Prodotti consigliati
Stato
liquid
Concentrazione
0.5% in isopropanol
tecniche
microbe id | staining: suitable
Colore
dark red
εmax
0.20-0.50 at 510-517 nm
applicazioni
diagnostic assay manufacturing
hematology
histology
Temperatura di conservazione
room temp
Stringa SMILE
N(\N=C3\c4c(cccc4)C=CC\3=O)c1c(cc(c(c1)C)N=Nc2c(ccc(c2)C)C)C
InChI
1S/C26H24N4O/c1-16-9-10-17(2)22(13-16)27-28-23-14-19(4)24(15-18(23)3)29-30-26-21-8-6-5-7-20(21)11-12-25(26)31/h5-15,29H,1-4H3/b28-27?,30-26-
HJAYEODIXUYVIC-LTFAAIOASA-N
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Categorie correlate
Descrizione generale
Applicazioni
Oil red O is a lysochrome (fat-soluble dye) diazo dye used for staining of neutral triglycerides and lipids on frozen sections and some lipoproteins on paraffin sections. In histology, a supersaturated solution of oil red O in isopropanol may be used to stain fat in tissue.
Avvertenze
Danger
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Eye Irrit. 2 - Flam. Liq. 2 - STOT SE 3
Organi bersaglio
Central nervous system
Codice della classe di stoccaggio
3 - Flammable liquids
Classe di pericolosità dell'acqua (WGK)
WGK 2
Punto d’infiammabilità (°F)
53.6 °F - closed cup
Punto d’infiammabilità (°C)
12.0 °C - closed cup
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Hi! I'm wondering if OilRedO is light sensitive? Does the incubation need to take place in the dark? And could the staining of cells be compromized if the solution is not kept dark?
1 answer-
Helpful?
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What are the differences between Oil Red O solution O1391 and OIL RED O staining solution 1.02419?
1 answer-
There are not many differences between products O1391 and 102419. O1391 is available in 250 mL and 500 mL bottles, while 102419 is available only in a 250 ml bottle. The only practical difference between the two products is the solvents used to dissolve the Oil Red O. For O1391, the solvent used is isopropyl alcohol, while the solvents used for 102419 are acetone and ethyl alcohol. Both products use Oil Red O as the dye, and the directions for use call for diluting the product with water prior to use and then filtering. The results should be identical since both products use Oil Red O as the dye.
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Should cells or tissues be fixed before staining with Oil Red O for fats or lipids?
1 answer-
Yes, cells or tissues need to be fixed before staining with Oil Red O. It is important to avoid using fixatives that contain alcohol. Oil Red O staining can be performed on cultured cells and fresh frozen tissue. However, tissues should not be paraffin embedded and exposed to the alcohols and solvents necessary for paraffin processing tissue.
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Hi I purchased Oil Red O solution – 0.5% in isopropanol, 01391 250mL, PCode-1003585017, Source, SLCP2956 for staining of whole mice aorta. I want to dilute it to 0.16% solution. What solution should I use for dilution? MilliQ water/methanol/isopropanol?
1 answer-
This product can be further diluted using distilled water or aqueous buffer to reach the desired concentration. Note that solution diluted with water or aqueous buffers are less stable and should not be stored for extended periods.
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Can I use this solution for stain frozen sections ? I thought that I bought oil red in propylene glycol. What is the difference ?
1 answer-
This O1391 is a 0.5% solution in isopropanol. Product O1516 is a 0.5% solution in propylene glycol. The protocol for either product is the same. There is some evidence in the literature that the propylene glycol solution performs better on PFPP samples, however this is not definitive and may depend on conditions and technique. Each is considered a stock concentration. Stock solutions can range from 0.2 - 0.5%. Depending on the specific application the stock solution will need to be further diluted by 40 - 60%. For example, in staining frozen sections, 30ml of the O1391 would be diluted with 20ml of distilled water to reach a working concentration. Note that solution diluted with water or aqueous buffers are less stable and should not be stored for extended periods.
Please see the link below to review and article that may be helpful:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3449473/pdf/10616_2004_Article_3903.pdfHelpful?
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How do you prepare the working solution using this reagent? or is it already a prepared working solution?
1 answer-
This product is a 0.5% solution in isopropanol and is considered a stock concentration. Stock solution can range from 0.2 - 0.5%. Depending on the specific application the stock solution will need to be further diluted by 40 - 60%. For example, in staining frozen sections, 30ml of the O1391 would be diluted with 20ml of distilled water to reach a working concentration. Note that solution diluted with water or aqueous buffers are less stable and should not be stored for extended periods.
Helpful?
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