This kit requires the use of white flat-bottom 96-well plates. T1 and T2 values are solely dependent on the assay and plate set up. The T1 and T2 should be chosen within the linear range of the kinetic plot of the standard or samples. See the link below for a reference that may be useful.
https://pubmed.ncbi.nlm.nih.gov/36009234/
MAK378
Angiotensin II Converting Enzyme (ACE2) Inhibitor Screening Kit
100 fluorometric tests
Sinonimo/i:
ACE2 Inhibitor Discovery Kit
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About This Item
Codice UNSPSC:
12161503
NACRES:
NA.84
Prezzi e disponibilità al momento non sono disponibili
Prodotti consigliati
impiego
100 fluorometric tests
applicazioni
pharmaceutical
Metodo di rivelazione
fluorometric
Malattie correlate
immunological diseases; cardiovascular diseases; genitourinary disorders; pulmonary disorders
Temperatura di conservazione
−20°C
Descrizione generale
Angiotensin II converting enzyme (ACE2), a carboxypeptidase, is part of the renin-angiotensin system (RAS) that controls regulation of blood pressure by cleaving the C-terminal dipeptide of Angiotensin II to convert it into Angiotensin 1-7. It also cleaves Angiotensin I to produce Ang 1-9, of unknown function. ACE2 is a receptor of human coronaviruses, such as SARS and HCoV-NL63. It is expressed on the vascular endothelial cells of heart and kidney. The inhibitors of ACE2 could be able to regulate hypertension by changing vascular permeability. Screening for small molecule and peptide inhibitors might also help in finding treatment for coronavirus mediated infection.
Caratteristiche e vantaggi
The Angiotensin II Converting Enzyme (ACE2) Inhibitor Screening Kit is compatible with high-throughput handling systems.
Compatibilità
The kit is suitable for Screening/characterizing inhibitors/ligands of ACE2.
Principio
The Angiotensin II Converting Enzyme (ACE2) Inhibitor Screening Kit can be used to screen for potent inhibitors of ACE2 activity, it utilizes the ability of an active ACE2 to cleave a synthetic MCA based peptide substrate to release a free fluorophore. The released MCA can be easily quantified using a fluorescence microplate reader. In the presence of an ACE2 specific inhibitor, the enzyme loses its peptidase activity which results in decrease of fluorescence intensity.
Altre note
For additional information on our range of Biochemicals, please complete this form.
Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 2
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1. For fluorescence measurements, it is supposed to use White flat-bottom 96-well plates or Black flat-bottom 96-well plates? 2. What are the considerations for selecting two time points (T1 & T2) in the linear range of the plot?
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