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MAK157

Sigma-Aldrich

Renin Assay Kit

Renin Assay Kit
1 of 1 reviewers received a sample product or took part in a promotion

sufficient for 100 fluorometric tests

Sinonimo/i:

Renin Enzyme Detection Kit

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CHF 283.00

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1 KIT
CHF 283.00

About This Item

Codice UNSPSC:
12161503
NACRES:
NA.84

CHF 283.00

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impiego

sufficient for 100 fluorometric tests

Metodo di rivelazione

fluorometric

Malattie correlate

cardiovascular diseases

Temperatura di conservazione

−20°C

Informazioni sul gene

human ... REN(5972)
rat ... REN(24715)

Categorie correlate

Descrizione generale

Renin, also known as angiotensinogenase, is an aspartyl protease that cleaves angiotensinogen to form angiotensin I, the initial and rate-limiting step of the renin-angiotensin system (RAS). This system plays a critical role in the regulation of blood pressure and fluid balance. Increased renin activity can result in hypertension due to increased vasoconstriction and fluid retention. Inhibitors of renin can be used as a treatment for hypertension.[1]

Applicazioni

Renin Assay Kit has been used to determine the activity of renin.[2][3]

Compatibilità

The Renin Assay Kit provides a convenient and simple method for screening renin activity in a variety of samples or for screening renin inhibitors.

Principio

Renin activity is determined using a proprietary fluorescence resonance energy transfer (FRET) peptide containing a fluorescence quencher. Cleavage of the peptide by renin results in the production of a fluorescent product (λex = 540/λem = 590 nm). This assay is ~50-fold more sensitive than the EDANS/DABCYL-based assay.

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

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Certificati d'analisi (COA)

Lot/Batch Number
3760462
3760462
3510103
3181891
304044

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Aliskiren decreases oxidative stress and angiogenic markers in retinal pigment epithelium cells.
Sim?o S, et al.
Angiogenesis, 20(1), 175-181 (2017)
The hormone FGF21 stimulates water drinking in response to ketogenic diet and alcohol.
Song P, et al.
Cell Metabolism, 27(6), 1338-1347 (2018)
Árpád Kovács et al.
American journal of physiology. Heart and circulatory physiology, 310(11), H1671-H1682 (2016-04-10)
Hypertension (HTN) is a major risk factor for heart failure. We investigated the influence of HTN on cardiac contraction and relaxation in transgenic renin overexpressing rats (carrying mouse Ren-2 renin gene, mRen2, n = 6). Blood pressure (BP) was measured.
Caroline G Shimoura et al.
Journal of applied physiology (Bethesda, Md. : 1985), 128(5), 1329-1337 (2020-04-03)
Acute intermittent hypoxia (AIH) triggers sympathetic long-term facilitation (sLTF), a progressive increase in sympathetic nerve activity (SNA) linked to central AT1 receptor (AT1R) activation by circulating angiotensin II (ANG II). Here, we investigated AIH activation of the peripheral renin-angiotensin system
Yoshio T, et al.
Handbook of Hormones: Comparative Endocrinology for Basic and Clinical Research (2015)
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1 Review This action will navigate to reviews.

Questions

1–2 of 2 Questions  

  1. Species that this assay works on?

    1 answer

    1. Although the standard that has been tested is recombinant human renin, theoretically the kit should be suitable for all other species.

      Helpful?

  2. Does the kit MAK157 contain any Renin inhibitor? If so, how much Renin inhibitor should be added, and at what step of the assay should it be added in order to determine the Renin activity?

    1 answer

    1. The kit MAK157 does not contain any Renin inhibitor. To confirm the activity using this kit, researchers can use a RENIN INHIBITOR separately.The amount of inhibitor to use and the step to use the Renin inhibitor can be referred to from published literature.

      Helpful?

Reviews

1 of 1 reviewers received a sample product or took part in a promotion

Active Filters

  1. Oxford
    • Review 1
    • Vote 1
    2 out of 5 stars.

    Kit instructions misleading - be careful

    Kit provides you with 10mL assay buffer. At least 1.87mL needed for making standards. 5mL needed for making substrate. Leaves you with 3.13mL for diluting samples. If your samples need a lot of diluting this is insufficient. I did not find the customer service suggestion of using water to dilute my samples to be appropriate or helpful. They could instead just provide sufficient reagent. God help you if you want to run the assay twice, with two sets of standards, further depleting the assay buffer stock.

    Helpful?

    1. Response from MilliporeSigma:

      Thank you for taking the time to leave a review! We are sorry to hear that your experience did not meet expectations. We have passed on your feedback to the product manager, and would like to learn more about your experience to see if there is anything we can do to improve this assay kit. When you have a moment, please contact us by visiting https://www.sigmaaldrich.com/support/customer-support and submit a Report Product Issue ticket. We lo]ok forward to hearing from you soon!

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