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MAK228

Sigma-Aldrich

Calpain Activity Fluorometric Assay Kit

sufficient for 100 fluorometric tests

Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali


About This Item

Codice UNSPSC:
12352200

Metodo di rivelazione

fluorometric

Malattie correlate

endocrinological disorders, diabetes; neurological disorders; cancer; cardiovascular diseases

Temperatura di conservazione

−70°C

Descrizione generale

Activation of calpain is involved in many forms of physiological and pathological processes (e.g., apoptosis). Calpain activation requires cell membrane and Ca2+, and activated calpain is released into cytosol. The Calpain Activity Assay Kit provides optimized buffers and reagents for a convenient measurement of calpain activity.

Compatibilità

Suitable for analyzing calpain activity in apoptotic and other samples.

Principio

Calpain Activity Fluorometric Assay Kit is based on the cleavage of the calpain substrate, Ac-LLY-AFC. The Ac-LLY-AFC substrate emits blue light (max = 400 nm); upon cleavage by calpain, free AFC emits a yellow-green fluorescence ( max = 505 nm), which can be quantified using a fluorometer or a fluorescence plate reader. Comparison of the fluorescence intensity from a treated sample with a normal control allows determination of the changes in calpain activity.

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Warning

Indicazioni di pericolo

Classi di pericolo

Skin Sens. 1

Codice della classe di stoccaggio

10 - Combustible liquids


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