MAK162

Colorimetric Maleimide Assay Kit

sufficient for 100 colorimetric tests

• Codice UNSPSC: 12161503
• NACRES: NA.84

Proprietà

• impiego: sufficient for 100 colorimetric tests
• Metodo di rivelazione: colorimetric
• Malattie correlate: cancer
• Temperatura di conservazione: −20°C

Descrizione

Descrizione generale

Maleimide is an unsaturated imide[1] used in protein conjugation reactions.[2] The direct measurement of maleimide moieties following conjugation reactions by spectrophotometric analysis at 302 nm is insensitive due to the small extinction coefficient (620M-1 cm-1) and complicated by protein absorbance at this wavelength.

Compatibilità

Suitable for quantifying maleimide groups

Principio

The colorimetric maleimide quantiation kit provides a simple and direct procedure for measuring maleimide groups. The sample is first reacted with a known amount of excess thiol and then the remaining unreacted thiol is assayed using 4,4′-DTDP (4,4′-Dithiodipyridine). The amount of maleimide is calculated as the difference between the initial amount of thiol and the amount of unreacted thiol after the complete reaction of all maleimide groups.

Informazioni sulla sicurezza

• Pittogrammi: GHS07
• Avvertenze: Warning
• Indicazioni di pericolo: H302,H317,H319,H335
• Consigli di prudenza: P261 - P264 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338
• Classi di pericolo: Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Sens. 1 - STOT SE 3
• Organi bersaglio: Respiratory system
• Codice della classe di stoccaggio: 10 - Combustible liquids
• Punto d’infiammabilità (°F): 188.6 °F - closed cup
• Punto d’infiammabilità (°C): 87 °C - closed cup