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CS1070

Transglutaminase Assay Kit

sufficient for assays in two 96-well plates

Sinonimo/i:

TGases Assay Kit

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1 kit
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CHF 945.00

Informazioni su questo articolo

NACRES:
NA.28
UNSPSC Code:
12161503

CHF 945.00

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Quality Segment

200

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... TGM1(7051), TGM2(7052), TGM3(7053), TGM4(7047), TGM5(9333), TGM6(343641), TGM7(116179)

General description

The Transglutaminase Assay Kit is designed for the detection of transglutaminase activity in biological samples. The kit can also be used for screening of transglutaminase inhibitors/activators.

Application

The assay is based on transglutaminase catalysis of a covalent bond formation between the free amine group of Poly L-Lysine, which is covalently attached to the plate surface, and the γ-carboxamide group of biotin-TVQQEL-OH substrate present in the assay buffer. This reaction immobilizes biotin to the plate surface. The amount of immobilized biotin is proportional to the amount of active transglutaminase in the sample. The amount of immobilized biotin is determined using streptavidin-peroxidase and TMB substrate.

Biochem/physiol Actions

Transglutaminases (TGases) are a widely distributed and unique group of calcium dependent enzymes that catalyze the post-translational modification of proteins by the formation of isopeptide bonds. This occurs either through protein cross-linking via formation of γ-glutamyl-ε-lysine bonds or through incorporation of primary amines at selected peptide-bound glutamine residues. The cross-linked products, often of high molecular mass, are highly resistant to mechanical challenge and proteolytic degradation, and their accumulation is found in a number of tissues and processes, where such properties are important including skin, hair, blood clotting, and wound healing. Deregulation of the enzyme activity contributes to a number of human diseases.

Other Notes

The detection limit is 0.003 mU/assay or 0.03mU/ml of Transglutaminase.

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Questo articolo
WH0007052M10RAB1741SAB4502963
Transglutaminase Assay Kit sufficient for assays in two 96-well plates

Sigma-Aldrich

CS1070

Transglutaminase Assay Kit

Monoclonal Anti-TGM2 antibody produced in mouse clone 2F4, purified immunoglobulin, buffered aqueous solution

Sigma-Aldrich

WH0007052M10

Monoclonal Anti-TGM2 antibody produced in mouse

Human Transglutaminase 2 ELISA for serum, plasma and cell culture supernatants

Sigma-Aldrich

RAB1741

Human Transglutaminase 2 ELISA

Anti-Transglutaminase 2, N-Terminal antibody produced in rabbit affinity isolated antibody

Sigma-Aldrich

SAB4502963

Anti-Transglutaminase 2, N-Terminal antibody produced in rabbit

description

sufficient for assays in two 96-well plates

description

clone 2F4, purified immunoglobulin, buffered aqueous solution

description

for serum, plasma and cell culture supernatants

description

affinity isolated antibody

Gene Information

human ... TGM1(7051), TGM2(7052), TGM3(7053), TGM4(7047), TGM5(9333), TGM6(343641), TGM7(116179)

Gene Information

human ... TGM2(7052)

Gene Information

human ... TGM2(7052)

Gene Information

human ... TGM2(7052)

Quality Level

200

Quality Level

100

Quality Level

-

Quality Level

100

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

shipped in

dry ice

shipped in

dry ice

shipped in

wet ice

shipped in

wet ice

I componenti del kit sono disponibili anche separatamente

N° Catalogo
Descrizione
SDS & Determinazione del prezzo
  • T5398
    Transglutaminase from guinea pig liver, lyophilized powder, ≥1.5 units/mg protein 2 ea
    SDS
  • T0440
    3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System for ELISA, peroxidase substrate
    SDS
  • S5512
    Streptavidin−Peroxidase from Streptomyces avidinii, lyophilized powder 100 μg
    SDS

pictograms

Health hazardCorrosion
GHS08,GHS05

signalword

Danger

Hazard Classifications

Aquatic Chronic 3 - ED ENV 1 - Eye Dam. 1 - Met. Corr. 1 - Resp. Sens. 1 - Skin Corr. 1B

Classe di stoccaggio

8A - Combustible corrosive hazardous materials

wgk

WGK 3

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Certificati d'analisi (COA)

Lot/Batch Number

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Questions

1–2 of 2 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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