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MAK142

Sigma-Aldrich

Fluorometric Intracellular Ros Kit

sufficient for 200 fluorometric tests (Deep red)

Sinonimo/i:

Intracellular ROS Fluorescence Assay

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1 KIT
CHF 283.00

CHF 283.00

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1 KIT
CHF 283.00

About This Item

Numero CE:
200-664-3
Codice UNSPSC:
12161503
NACRES:
NA.25

CHF 283.00

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impiego

sufficient for 200 fluorometric tests (Deep red)

applicazioni

cosmetics
food and beverages

Metodo di rivelazione

fluorometric

Malattie correlate

neurological disorders; cancer

Temperatura di conservazione

−20°C

Categorie correlate

Descrizione generale

Reactive oxygen species (ROS) are generated as a result of the reduction of oxygen during aerobic respiration and by various enzymatic systems within the cell. At physiological levels, ROS contribute to cell signaling and host defense.[1] Increased ROS generation, above the detoxification capacity of the biological system, results in oxidative stress and cellular damage.[2] The main damage to cells results from the ROS-induced alteration of macromolecules such as polyunsaturated fatty acids in membrane lipids, essential proteins, and DNA. ROS has been implicated in disease states, such as Alzheimer′s disease, Parkinson′s disease, cancer, and aging.[3]

Applicazioni


  • Diethyl Blechnic Exhibits Anti-Inflammatory and Antioxidative Activity via the TLR4/MyD88 Signaling Pathway in LPS-Stimulated RAW264.7 Cells.: This article uses fluorometric methods to explore the anti-inflammatory and antioxidative properties of diethyl blechnic, illustrating the role of ROS assays in elucidating the molecular mechanisms underlying the therapeutic effects of natural compounds (Yang S et al., 2019).

Compatibilità

This kit is suitable for the detection of intracellular ROS by fluorescence microplate reader, flow cytometer, or a fluorescent microscope with TRITC filter.

Principio

The Fluorometric Intracellular ROS Kit provides a sensitive, one-step fluorometric assay to detect intracellular ROS (especially superoxide and hydroxyl radicals) in live cells after 1 hour incubation. ROS react with a fluorogenic sensor localized to the cytoplasm, resulting in a fluorometric product proportional to the amount of ROS present. The assay can be performed in either a 96 or 384 multiwell plate format and with detection at λex = 650/λem = 675 nm.

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

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Certificati d'analisi (COA)

Lot/Batch Number
3510351
3510351
3181881
3181881
2781712

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Bovine herpesviruses induce different cell death forms in neuronal and glial-derived tumor cell cultures.
Cardoso T C, et al.
Journal of Neurovirology, 22(6), 725-735 (2016)
The role of reactive oxygen species in the pathogenesis of Alzheimer?s disease, Parkinson?s disease, and Huntington?s disease: a mini review.
Manoharan S, et al.
Oxidative Medicine and Cellular Longevity, 2016 (2016)
Mitochondrial bioenergy alterations in avian HD11 macrophages infected with infectious bronchitis virus.
da Silva S E L, et al.
Archives of Virology, 1-7 (2018)
Sergio E L da Silva et al.
Archives of virology, 163(4), 1043-1049 (2018-01-06)
To establish an association between mitochondrial dysfunction and apoptosis following infectious bronchitis virus (IBV) infection, HD11 avian macrophage cells were infected with the Massachusetts 41 (M41) strain. Our results show that the M41 strain of IBV induced cytopathic effects followed
Tereza C Cardoso et al.
Journal of neurovirology, 22(6), 725-735 (2016-06-18)
Oncolytic viruses have the ability to infect tumor cells and leave healthy cells intact. In this study, bovine herpesvirus 1 (BHV1; Los Angeles, Cooper, and SV56/90 strains) and bovine herpesvirus 5 (BHV5; SV507/99 and GU9457818 strains) were used to infect
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Questions

1–2 of 2 Questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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