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MAK132

Sigma-Aldrich

Sulfate Assay Kit

sufficient for 200 tests (turbidimetric)

Sinonimo/i:

Sulfate Determination Kit

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1 KIT
CHF 419.00

CHF 419.00


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1 KIT
CHF 419.00

About This Item

Codice UNSPSC:
12161503
NACRES:
NA.84

CHF 419.00


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impiego

sufficient for 200 tests (turbidimetric)

Metodo di rivelazione

turbidimetric

Malattie correlate

genitourinary disorders

Temperatura di conservazione

room temp

Descrizione generale

Inorganic sulfate is one of the most abundant anions in mammalian plasma. Sulfate plays important physiological roles in activating and detoxifying xenobiotics, steroids, neurotransmitters, and bile acids.[1] Sulfate is needed for the biosynthesis of cerebroside sulfate, glycosaminoglycans, and heparin sulfate. Undersulfation of cartilage proteoglycans has been associated with human inherited osteochondro-dysplasia disorders.[2] In mammals, sulfate homeostasis is regulated by the kidney. The majority of filtered sulfate is absorbed in the proximal tubules and only 5-20% of the filtered load is excreted into the urine.[3][4]

Applicazioni

Sulfate Assay Kit has been used to determine the concentration of sulfate.[5]

Caratteristiche e vantaggi

Compatible with high-throughput handling systems. Can be adapted for use with cuvettes.

Compatibilità

Suitable for the detection of sulfate in biological fluids such as serum and urine.

Principio

The kit employs an improved method utilizes the quantitative formation of insoluble barium sulfate in polyethylene glycol. The turbidity measured at 600 nm is proportional to sulfate level in the sample.

Avvertenze

Danger

Indicazioni di pericolo

Classi di pericolo

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Corr. 1A - STOT SE 3

Organi bersaglio

Respiratory system

Codice della classe di stoccaggio

8A - Combustible corrosive hazardous materials


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1 of 3

Physiological Roles and Regulation of Mammalian Sulfate Transporters.
Daniel M.
Physiological Reviews, 81(4) (2001)
Transcriptional regulation of the sodium-sulfate cotransporter NaSi-1 gene.
Dawson P A and Daniel M
Cell Biochemistry and Biophysics, 36(2-3), 175-182 (2002)
Niklas Wahlström et al.
Carbohydrate polymers, 233, 115852-115852 (2020-02-16)
The cell wall polysaccharide ulvan was isolated from two species of the seaweed Ulva collected along the Swedish west coast. Acidic extraction was benchmarked against hot water extraction with enzymatic purification and against commercial ulvan. Extracted ulvan contained 11-18 %
Esters production via carboxylates from anaerobic paper mill wastewater treatment.
Carlos I C R, et al.
Bioresource Technology, 237 , 186-192 (2017)
Molecular aspects of renal tubular handling and regulation of inorganic sulfate.
Beck L and Caroline S
Kidney International, 59(3), 835-845 (2001)

Questions

1–2 of 2 Questions  
  1. Can the kit be applied to automated biochemical analysers?

    1 answer
    1. This kit has not been specifically tested with automated biochemical analyzers. The current protocol is designed for manual use; however, there is no indication that the kit cannot be used with automated biochemical analyzers.

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  2. Can the MAK132 Sulfate assay kit be used to test wine instead of plasma? If so, are there any recommended protocol adjustments for this?

    1 answer
    1. The MAK132 assay kit is designed for testing sulfates, not sulfites, which are more commonly associated with wine. There is no available data on this kit being previously tested with wines. If the wine sample is diluted and/or the color is reduced through treatment with carbon or polyvinylpyrrolidone (PVPP) and the sample sulfate levels are within the detection range of 0.02 mM (0.19 mg/dL) to 2 mM (19.2 mg/dL) in a 96-well plate assay, then it might work for testing wine instead of plasma.

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