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Merck

BCA1

Kit dell′acido bicinconinico per la determinazione delle proteine

for 200-1000 μg/ml protein

Sinonimo/i:

Saggio proteico BCA

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1 KIT

CHF 489.00

CHF 489.00

Spedizione prevista il17 aprile 2026Dettagli

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Informazioni su questo articolo

NACRES:
NA.32
UNSPSC Code:
12352106

Documenti fondamentali

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usage

sufficient for ~500 assays

storage temp.

2-8°C

Quality Level

200

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Questo articolo
QPBCAB964357697
Bicinchoninic Acid Kit for Protein Determination for 200-1000 μg/ml protein

Sigma-Aldrich

BCA1

Bicinchoninic Acid Kit for Protein Determination

QuantiPro™ BCA Assay Kit for 0.5-30 μg/ml protein

Sigma-Aldrich

QPBCA

QuantiPro™ BCA Assay Kit

Bicinchoninic Acid solution

Supelco

B9643

Bicinchoninic Acid solution

Sigma-Aldrich

Sigma-Aldrich

57697

Advanced Protein Assay

usage

sufficient for ~500 assays

usage

-

usage

-

usage

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

-

storage temp.

2-8°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

Application

Il kit dell′acido bicinconinico è stato utilizzato per la stima delle proteine nei campioni di proteomi salivari, nelle cellule di rene embrionale umano 293 e nelle cellule luteiniche.

Biochem/physiol Actions

Le proteine riducono il Cu(II) alcalino in Cu(I) in modo dipendente dalla concentrazione. L′acido bicinconinico è un reagente cromogenico altamente specifico per il Cu(I) che forma un complesso di colore viola con un′assorbanza massima a 562 nm. L′assorbanza è direttamente proporzionale alla concentrazione di proteine. È un′alternativa al reagente di Folin-Ciocalteu per la determinazione delle proteine.

Features and Benefits

  • Saggio colorimetrico semplice e sensibile per le proteine
  • Due reagenti stabili utilizzati per la soluzione di lavoro
  • Un metodo più semplice e rapido del saggio proteico di Lowry
  • Compatibile con molti detergenti ionici e non ionici
  • Minore variazione tra diverse proteine rispetto al saggio sul legame con il colorante di Bradford
  • Adattabile all′uso su piastre con micropozzetti

Other Notes

Vincitore del CiteAb Award 2024 per i fornitori che hanno conseguito risultati nella ricerca sul Parkinson

Solo come componenti del kit

N° Catalogo
Descrizione
  • Bicinchoninic Acid Solution 1 L
  • 4%(w/v) CuSO4 · 5H2O Solution 25 mL
  • Protein Standard Solution 5 x 1

used together

N° Catalogo
Descrizione
Determinazione del prezzo

pictograms

CorrosionEnvironment
GHS05,GHS09

signalword

Danger

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Met. Corr. 1

Classe di stoccaggio

8A - Combustible corrosive hazardous materials

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

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Certificati d'analisi (COA)

Lot/Batch Number
0000502790
0000502807
0000502807
0000502790
0000484749

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Salivary proteome patterns of individuals exposed to High Altitude
Jain S, et al.
Archives of Oral Biology, 96, 104-112 (2018)
D Jantas et al.
Neurotoxicity research, 25(2), 208-225 (2013-10-10)
Tianeptine (Tian) possesses neuroprotective potential, however, little is known about the effect of this drug in models of neuronal apoptosis. In the present study, we aimed (1) to compare the neuroprotective capacities of some antidepressants (ADs) in the models of
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Analytical biochemistry, 180(1), 136-139 (1989-07-01)
Protein quantitation based on bicinchoninic acid (BCA) is simple, sensitive, and tolerant to many detergents and substances known to interfere with the Lowry method. However, certain compounds often used during protein purification do interfere with the BCA protein assay. The
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Articoli

Determination of Protein by Bicinchoninic Acid Assay

BCA protein assay or Smith assay to determine protein concentration in a sample using Spectroquant® Prove photometers.

Contenuto correlato

Questions

1–4 of 4 Questions  

  1. Good morning. Is the "Bicinchoninic Acid Kit for Protein Determination" complete? i.e. does it contain all the necessary reactants? Is there a need to buy plates, wells or other? Thank you.

    1 answer

    1. This kit does require the use of materials that are dependant on the assay format used, but not provided in the kit. These items are as follows:

      • Spectrophotometer capable of accurately measuring absorbance in the 560 nm region
      • 96-well plates, such as Cat. No. CLS9017
      • 96-well plate sealing film: Cat. No. Z369667

      OR

      • Test tubes, 13 × 100 mm: Cat. No. CLS980013
      • 1 mL Disposable Plastic Cuvettes: Cat. No. C5416

      Please see the link below to review additional information available in the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/246/793/bca1pis-ms.pdf

      Helpful?

  2. Can the kit be used for measuring the protein content of marine bacteria grown in artificial sea water?

    1 answer

    1. This product has not been tested for use with marine bacteria in artificial sea water. However, the publication below used the Bicinchoninic acid method to measure total protein in planktonic marine bacteria, indicating that this kit may be suitable.
      Determination of Total Protein Content of Bacterial Cells by SYPRO Staining and Flow Cytometry
      https://www.ncbi.nlm.nih.gov/pmc/articles/PMC91485/

      Note that there are a number of potential interfering substances that may impact results. Please see the link below to review the product datasheet, including a list of interfering substances beginning on page 4.
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/246/793/bca1pis-ms.pdf

      Helpful?

  3. Can a standard OD plate reader be used for the BCA1-1KT Kit, which offers a choice of assay tubes and 96-well plates as mentioned in the technical description? Additionally, would the Test tubes, 13 × 100 mm, Catalog Number CLS980013 not be needed?

    1 answer

    1. If a standard ELISA plate reader capable of measuring absorbance in the 560nm region is being used, the 96-well format should be suitable. The test tubes are recommended for larger volume analysis with cuvettes, but if the 96-well format is used, they will not be needed for the samples. However, they are also being utilized to make the BSA protein standard curve dilutions. If there is an alternative method for making the serial standard protein dilutions that ensures thorough mixing and utilizes a low-binding surface to prevent protein absorption, such as borosilicate glass and/or polypropylene tubes, that will be suitable as well.

      Helpful?

  4. Does this kit rely on naked-eye colorimetric detection or does it require a fluorescent microscope?

    1 answer

    1. The kit is based on colorimetric, not fluorometric, detection. The color intensity should be measured at A562nm

      Helpful?

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