MAK122
Phospholipid Assay Kit
sufficient for 100 colorimetric or fluorometric tests
Sinonimo/i:
Phospholipid Quantification Kit
Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali
About This Item
Codice UNSPSC:
12161503
NACRES:
NA.84
Prodotti consigliati
impiego
sufficient for 100 colorimetric or fluorometric tests
applicazioni
cosmetics
food and beverages
pharmaceutical
Metodo di rivelazione
colorimetric
fluorometric
Condizioni di spedizione
dry ice
Temperatura di conservazione
−20°C
Categorie correlate
Descrizione generale
Phospholipids are a class of lipids which constitute a major component of cell membranes and play important roles in signal transduction. Most phospholipids contain one diglyceride, a phosphate group, and one choline.
In this assay, phospholipids (such as lecithin, lysolecithin and sphingomyelin) are enzymatically hydrolyzed to choline which is determined using choline oxidase and a H2O2-specific dye. This results in a colorimetric (570 nm)/ fluorometric (λex = 530/λem = 585 nm) product directly proportional to the phospholipid concentration in the sample. The range of linear detection is 3 - 200 μM for colorimetric assays and 0.6 - 20 μM for fluorometric assays.
In this assay, phospholipids (such as lecithin, lysolecithin and sphingomyelin) are enzymatically hydrolyzed to choline which is determined using choline oxidase and a H2O2-specific dye. This results in a colorimetric (570 nm)/ fluorometric (λex = 530/λem = 585 nm) product directly proportional to the phospholipid concentration in the sample. The range of linear detection is 3 - 200 μM for colorimetric assays and 0.6 - 20 μM for fluorometric assays.
Applicazioni
Phospholipid assay kit has been used in biochemical analysis and phospholipid quantitation.
Caratteristiche e vantaggi
Compatible with high-throughput handling systems.
Compatibilità
Suitable for the detection of phospholipids in biological samples
Principio
In this assay, phospholipids (such as lecithin, lysolecithin and sphingomyelin) are enzymatically hydrolyzed to choline which is determined using choline oxidase and a H2O2-specific dye. This results in a colorimetric (570 nm)/ fluorometric (λex = 530/λem = 585 nm) product directly proportional to the phospholipid concentration in the sample. The range of linear detection is 3 - 200 μM for colorimetric assays and 0.6 - 20 μM for fluorometric assays.
Avvertenze
Danger
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Aquatic Chronic 3 - Resp. Sens. 1
Codice della classe di stoccaggio
10 - Combustible liquids
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