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MAK094

Sigma-Aldrich

Protein Carbonyl Content Assay Kit

sufficient for 100 colorimetric tests

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About This Item

Codice UNSPSC:
12161503
NACRES:
NA.84

impiego

sufficient for 100 colorimetric tests

Metodo di rivelazione

colorimetric

Malattie correlate

cardiovascular diseases; neurological disorders; cancer; gastrointestinal diseases

Temperatura di conservazione

2-8°C

Descrizione generale

Oxidative stress results when the effectiveness of antioxidant defenses is insufficient to deal with the production of reactive oxygen species (ROS). ROS can induce damage to DNA, lipids, and proteins. The oxidation of proteins results in the production of stable carbonyl groups, which can be used as a measure of oxidative injury.

Applicazioni

Protein carbonyl content assay kit has been used to determine protein carbonyl concentration.

Compatibilità

Suitable for quantifying carbonyls in protein samples

Principio

The Protein Carbonyl Content Assay Kit provides a simple and direct procedure for measuring carbonyl content in a variety of biological samples. Carbonyl content is determined by the derivatization of protein carbonyl groups with 2,4-dinitrophenylhydrazine (DNPH) leading to the formation of stable dinitrophenyl (DNP) hydrazone adducts, which can be detected spectrophotometrically at 375 nm, proportional to the carbonyls present.

Sostituito da

N° Catalogo
Descrizione
Determinazione del prezzo

Avvertenze

Danger

Indicazioni di pericolo

H290,H302,H314,H335,H341,H351,H410

Classi di pericolo

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Dam. 1 - Met. Corr. 1 - Muta. 2 - Skin Corr. 1 - STOT SE 3

Organi bersaglio

Respiratory system

Codice della classe di stoccaggio

8A - Combustible corrosive hazardous materials

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Effects of chilled-then-frozen storage (up to 52 weeks) on an indicator of protein oxidation and indices of protein degradation in lamb M. longissimus lumborum.
Coombs CE, et al.
Meat Science, 135, 134-141 (2018)
Melissa M Miranda-Cruz et al.
Comparative biochemistry and physiology. Toxicology & pharmacology : CBP, 213, 19-26 (2018-07-25)
Hypoxia inducible factor-1 (HIF-1) is a transcriptional factor that induces genes involved in glucose metabolism. HIF-1 is formed by a regulatory α-subunit (HIF-1α) and a constitutive β-subunit (HIF-1β). The white spot syndrome virus (WSSV) induces a shift in glucose metabolism
Benjamin W B Holman et al.
Meat science, 139, 171-178 (2018-02-11)
Different chilled (~0.1 °C for up to 5 weeks) then frozen storage (up to 12 months) combinations and two frozen storage holding temperatures (-12 °C and -18 °C) effects on beef M. longissimus lumborum (LL) protein structure degradation and a marker of protein oxidation were
UV?B radiation interacts with temperature to determine animal performance.
Ghanizadeh-Kazerouni E, et al.
Functional Ecology, 30(4), 584-595 (2016)
Yujie Huang et al.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 49(2), 758-779 (2018-08-31)
Skeletal muscle atrophy is an important health issue and can impose tremendous economic burdens on healthcare systems. Glucocorticoids (GCs) are well-known factors that result in muscle atrophy observed in numerous pathological conditions. Therefore, the development of effective and safe therapeutic
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