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M7403

MCDB 153 Medium

With trace elements, L-glutamine and 28mM HEPES, without sodium bicarbonate, powder, suitable for cell culture

Sinonimo/i:

Molecular, Cellular, and Development Biology 153 medium

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Informazioni su questo articolo

NACRES:
NA.75
UNSPSC Code:
12352207
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Quality Segment

form

powder

technique(s)

cell culture | mammalian: suitable

components

NaHCO3: no
L-glutamine: 0.8772 g/L
glucose: 1.081 g/L (Dextro)
sodium pyruvate: 0.055 g/L
phenol red: 0.001242 g/L
HEPES: 6.6 g/L (25mM)

shipped in

ambient

storage temp.

2-8°C

General description

MCDB 153 media are modifications of Ham′s nutrient mixture F-12, designed for the growth of human keratinocytes, clonal growth of chicken embryo fibroblasts, and Chinese hamster ovary (CHO) cells using low levels of fetal bovine serum protein (FBSP), extensive trace elements or no serum protein.

Application

MCDB 153 Medium has been used as a component of melanoma isolation media (MIM) to culture human non-metastatic and metastatic melanoma cells.

Preparation Note

Formulated to contain 17.6 grams of powder per liter of medium.
Supplement with 1.18 g/L sodium bicarbonate.

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Sigma-Aldrich

Sigma-Aldrich

M7403

MCDB 153 Medium

MCDB 131 Medium With trace elements and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Sigma-Aldrich

M8537

MCDB 131 Medium

Sigma-Aldrich

Sigma-Aldrich

M6770

MCDB 201 Medium

Sigma-Aldrich

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M6395

MCDB 105 Medium

description

With trace elements, L-glutamine and 28mM HEPES, without sodium bicarbonate, powder, suitable for cell culture

description

With trace elements and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

description

With trace elements, L-glutamine and 30 mM HEPES, powder, suitable for cell culture

description

With trace elements, L-glutamine and 25mM HEPES, powder, suitable for cell culture

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

form

powder

form

powder

form

powder

form

powder

components

NaHCO3: no
L-glutamine: 0.8772 g/L
glucose: 1.081 g/L (Dextro)
sodium pyruvate: 0.055 g/L
phenol red: 0.001242 g/L
HEPES: 6.6 g/L (25mM)

components

sodium pyruvate: 0.11 g/L
phenol red: 0.012 g/L
L-glutamine: 1.461 g/L
HEPES: no
glucose: 1.0 g/L (Dextro)

components

HEPES: 7.149 g/L (30mM)
glucose: 1.441 g/L (Dextro)
phenol red: 0.001242 g/L
sodium pyruvate: 0.055 g/L
L-glutamine: 0.14615 g/L

components

phenol red: 0.001242 g/L
glucose: 0.72064 g/L (Dextro)
sodium pyruvate: 0.11 g/L
HEPES: 5.958 g/L (25mM)
L-glutamine: 0.3653 g/L

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

Quality Level

500

Quality Level

-

Quality Level

-

Quality Level

500


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Classe di stoccaggio

11 - Combustible Solids

wgk

WGK 1

flash_point_f

No data available

flash_point_c

No data available



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Questions

1–2 of 2 Questions  
  1. What is the recommended protocol for co-culturing keratinocytes, fibroblasts, and endothelial cells using MCDB153 media?

    What is the recommended protocol for co-culturing keratinocytes, fibroblasts, and endothelial cells using MCDB153 media?

    1 answer
    1. Additionally, an extensive literature search revealed an article discussing the effect of type IV collagen on fibrin assembly kinetics. The authors indicated that as the concentration of collagen increased from 0 to 100 ng/ml, the rate of fibrin assembly increased, as evidenced by an increase in the initial slope seen in Fig. 1. The article can be found at https://www.jbc.org/content/263/15/7043.full.pdf. It may be helpful to use similar guidelines.

      Helpful?

  2. 이 용액으로 배양한 세포를 인체에 투여하는 것이 가능한가요?

    1 answer
    1. Unless otherwise stated, this product is intended for research use only and is not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses, or any type of consumption or application to humans or animals.

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