The solubility in water has not been evaluated for these products. It is recommended to follow the reconstitution instructions and reconstitute at a concentration of up to 20 mg/mL in 1 M NaOH.
M2378
Mucina
Type II
Sinonimo/i:
MUC
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Cambia visualizzazione
| A voi/SKU | Disponibilità | Prezzo |
|---|---|---|
100 g | Per conoscere la disponibilità, visualizza il carrello | CHF 207.00 |
500 g | Per conoscere la disponibilità, visualizza il carrello | CHF 748.00 |
Informazioni su questo articolo
Form:
powder
Biological source:
Porcine stomach
Servizio Tecnico
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Permettici di aiutartibiological source
Porcine stomach
Quality Segment
type
Type II
form
powder
composition
bound sialic acid, ≤1.2%
technique(s)
cell culture | mammalian: suitable
solubility
1 M NaOH: soluble 20 mg/mL
UniProt accession no.
storage temp.
2-8°C
Gene Information
pig ... MUC2(100127145)
General description
La mucina è composta principalmente da unità di carboidrati, cruciali per l′architettura strutturale e la conformazione. Il monomero della mucina corrisponde a 640 kDa e le proteine contribuiscono soltanto al 20% del peso molecolare. La componente di carboidrati è legata alle catene laterali di treonina e serina. Le mucine sono classificate in mucine secretorie e mucine transmembrana.
Application
La mucina da stomaco suino è stata utilizzata:
- nella generazione della curva standard per la quantificazione della mucina-2 generata in condizioni di infiammazione della mucosa nei topi
- come componente di soluzioni liquide di digestione in modelli di digestione umana in vitro[3]
- come componente di un fluido simulante la saliva
La mucina da stomaco porcino è stata utilizzata in studi sul sito di legame dell′agglutinina PA-IL da Pseudomonas aeruginosa specifica per il galattosio.[4]
Biochem/physiol Actions
La mucina trova ampio impiego in applicazioni farmacologiche di somministrazione dei farmaci. Le mucine svolgono un ruolo protettivo nell′infiammazione. Lo sregolamento della sintesi delle mucine contribuisce alla tumorigenesi e all′infiammazione cronica.
Il muco forma uno strato protettivo che riveste tutte le superfici epiteliali. I principali componenti strutturali del muco sono le mucine gelificanti. Lo strato di muco che ricopre l′epitelio intestinale contiene come molecola centrale la mucina MUC2. La MUC2 è prodotta dalle cellule caliciformi.[5]
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Questo articolo | |||
|---|---|---|---|
| biological source Porcine stomach | biological source Porcine stomach | biological source - | biological source Porcine |
| technique(s) cell culture | mammalian: suitable | technique(s) microbiological culture: suitable | technique(s) - | technique(s) cell culture | mammalian: suitable |
| form powder | form partially purified powder | form lyophilized | form lyophilized powder |
| UniProt accession no. | UniProt accession no. - | UniProt accession no. - | UniProt accession no. |
| solubility 1 M NaOH: soluble 20 mg/mL | solubility NaOH: soluble 20 mg/mL | solubility dilute aqueous base buffer: soluble, water: soluble | solubility - |
| Gene Information pig ... MUC2(100127145) | Gene Information - | Gene Information - | Gene Information Porcine ... ALB(396960) |
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Classe di stoccaggio
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Which mucin is more water soluble M2378 or M1778 ?
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What is the best way to sterilise the product without destroying the structure?
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The structural integrity of this product has not been evaluated post-sterilization. A general procedure for sterilizing mucin has been published as follows:
1. Place dry powdered mucin in a flask.
2. Cover the mucin with 95% ethanol.
3. Heat the covered mucin at 70 °C for 24 hours.
4. Evaporate the alcohol to recover the mucin.
This information is taken from Yeung, A.T.Y. et al., MBio, 3(3), e00073-12 (2012).In addition, this product is described as soluble in 1 M NaOH: soluble 20 mg/mL:
https://www.sigmaaldrich.com/product/sigma/m2378This Millex syringe filter should be chemically compatible to filter sterilize that solution (this product is intended for a volume range ~ 10 – 100 ml solution):
https://www.sigmaaldrich.com/product/mm/slgvr33rsSee user guide:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/409/570/pr05510w-ms.pdfHelpful?
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How does this differ from human mucin?
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This product has not been tested in comparison to human-source mucin.
It may be helpful to read through the following publication:
https://www.nature.com/articles/s41598-020-77129-4Helpful?
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I saw that this product is made by peptide degradation. by that way, can it harvest transmembrane mucins? I wonder this product contains transmembrane mucin too, especially MUC1.
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This product is a very crude preparation of mucin obtained by digestion of hog stomach with pepsin, followed by precipitation of the mucin with alcohol. The crude mucin is collected by filtration and dried. It is a mixture of glycoproteins. Transmembrane mucins tend to be heavily glycosylated and are more resistant to digestion however, conditions such as pH, temperature, and activity of the enzyme may lead to degradation. Further characterization of the mucins present in the final product is not performed.
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What is the molecular weight of Product M2378, Mucin from porcine stomach?
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This product is a crude mixture of glycoproteins. The sugar and amino acid composition of the mucin has not been determined.
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How can I solubilize Product M2378, Mucin from porcine stomach?
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This crude mucin may not be readily soluble in solution. At 20 mg/mL in 1 M sodium hydroxide, turbid suspensions are observed. To try to increase the solubility, one might try further dilution and sonication, but we have not tried this in our labs.
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What are the differences between Mucin from porcine stomach Products M2378 and M1778?
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Product No. M2378 is a crude mucin preparation. Product No. M1778 is a partially purified powder prepared according to the method described in Glenister, D.A. and Salmon. K. Microbial Ecol. in Health & Disease 1, 31, (1988). The amount of bound sialic acids are also different. Please refer to the specification sheets on our website.
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What does the "Type" designation in Mucin from porcine stomach mean?
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The type designation does not have any particular biological significance. It is used to emphasize differences in the listings of this product.
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Is Product M2378, Mucin from porcine stomach, a purified preparation of mucin?
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No. This product is a crude preparation of mucin; it is a mixture of glycoproteins. It is obtained by digestion of hog stomach with pepsin, followed by precipitation and other steps.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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